PMID- 10674970
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20190826
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 54
IP  - 6
DP  - 1999 Dec
TI  - Significant associations of HLA-B*5101 and B*5108, and lack of association of 
      class II alleles with Behcet's disease in Italian patients.
PG  - 565-71
AB  - Behcet's disease has been known to be strongly associated with human leukocyte 
      antigen (HLA) B51, one of the split antigens of HLA-B5. An increased incidence of 
      HLA-B51 in the patient group has also been reported in an Italian population. 
      Since the B51 antigen has been recently identified to comprise nine alleles, 
      B*5101-B*5109, we performed HLA-B51 allele genotyping by the polymerase chain 
      reaction-sequencing based typing (PCR-SBT) method as well as serological HLA-A 
      and -B typing among 21 Italian patients with Behcet's disease in order to 
      investigate whether there is any correlation of one particular B51-associated 
      allele with Behcet's disease. In addition, HLA class II genotyping was performed 
      by the PCR-restriction fragment length polymorphism (RFLP) method. As a result, 
      only the phenotype frequency of the B51 antigen was found to be significantly 
      increased in the patient group as compared to the ethnically matched control 
      group by the corrected P-value analysis (71.4% in patients vs. 17.9% in controls; 
      chi2 = 14.26, Pc = 0.0042, R.R. = 11.5). In the B51 allele genotyping, 11 out of 
      15 B51-positive patients were B*5101 and the remaining four were B*5108, whereas 
      all of 5 normal controls were B*5101, showing significant association of each 
      allele with Behcet's disease. No significant difference was observed between the 
      patient and control groups in the HLA class II allelic distribution. This study 
      revealed a strong association of Behcet's disease in Italian with B*5108 as well 
      as B*5101, providing important insight into the molecular mechanism underlying an 
      HLA association with Behcet's disease.
FAU - Kera, J
AU  - Kera J
AD  - Department of Genetic Information, Tokai University School of Medicine, 
      Bohseidai, Isehara, Kanagawa, Japan.
FAU - Mizuki, N
AU  - Mizuki N
FAU - Ota, M
AU  - Ota M
FAU - Katsuyama, Y
AU  - Katsuyama Y
FAU - Pivetti-Pezzi, P
AU  - Pivetti-Pezzi P
FAU - Ohno, S
AU  - Ohno S
FAU - Inoko, H
AU  - Inoko H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Genetic Markers)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Alleles
MH  - Behcet Syndrome/epidemiology/*genetics/immunology
MH  - Genetic Markers
MH  - HLA-B Antigens/*genetics
MH  - Histocompatibility Antigens Class II/*genetics
MH  - Humans
MH  - Italy/epidemiology
EDAT- 2000/02/16 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/16 09:00
PHST- 2000/02/16 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/16 09:00 [entrez]
AID - 10.1034/j.1399-0039.1999.540605.x [doi]
PST - ppublish
SO  - Tissue Antigens. 1999 Dec;54(6):565-71. doi: 10.1034/j.1399-0039.1999.540605.x.