PMID- 10675609
OWN - NLM
STAT- MEDLINE
DCOM- 20000403
LR  - 20201208
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 243
IP  - 1-2
DP  - 2000 Feb 8
TI  - Chain reaction cloning: a one-step method for directional ligation of multiple 
      DNA fragments.
PG  - 19-25
AB  - A novel DNA assembly method, chain reaction cloning (CRC), is described. CRC 
      enables the ordered assembly of multiple DNA fragments in a single step. The 
      power of the technique was demonstrated by the directed in vitro assembly of a 
      plasmid comprised of six DNA fragments from a pool of 12 available fragments. The 
      odds of obtaining the correct plasmid clone in a single step, using conventional 
      techniques, is less than 1 in 191000000. Using CRC, the desired plasmid was 
      recovered at a frequency of one in two. Ligation is no longer the rate limiting 
      step in cloning, and limitless possibilities exist for the reconstruction of 
      complex genomes.
FAU - Pachuk, C J
AU  - Pachuk CJ
AD  - Wyeth Research, One Great Valley Parkway, Suite 30, Malvern, PA, USA. 
      pachukc@labs.wyeth.com
FAU - Samuel, M
AU  - Samuel M
FAU - Zurawski, J A
AU  - Zurawski JA
FAU - Snyder, L
AU  - Snyder L
FAU - Phillips, P
AU  - Phillips P
FAU - Satishchandran, C
AU  - Satishchandran C
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Oligonucleotides)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.7 (DNA Polymerase I)
RN  - EC 6.5.1.- (DNA Ligases)
SB  - IM
MH  - Cloning, Molecular/*methods
MH  - DNA/genetics/metabolism
MH  - DNA Ligases/metabolism
MH  - DNA Polymerase I/metabolism
MH  - Oligonucleotides/genetics
MH  - Plasmids
MH  - Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
EDAT- 2000/02/17 00:00
MHDA- 2000/02/17 00:01
CRDT- 2000/02/17 00:00
PHST- 2000/02/17 00:00 [pubmed]
PHST- 2000/02/17 00:01 [medline]
PHST- 2000/02/17 00:00 [entrez]
AID - S0378-1119(99)00508-9 [pii]
AID - 10.1016/s0378-1119(99)00508-9 [doi]
PST - ppublish
SO  - Gene. 2000 Feb 8;243(1-2):19-25. doi: 10.1016/s0378-1119(99)00508-9.