PMID- 10676853
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20220310
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 36
IP  - 3
DP  - 2000 Mar
TI  - Serotonin transporter function is modulated by brain-derived neurotrophic factor 
      (BDNF) but not nerve growth factor (NGF).
PG  - 197-202
AB  - The serotonin transporter (5-HTT) regulates serotonergic neurotransmission by 
      determining the magnitude and duration of serotonergic responses. We have 
      recently described a polymorphism in the 5-HTT gene promoter (5-HTTLPR) which 
      influences the function of the 5-HTT and is associated with several psychiatric 
      disorders. Immortalized B lymphocytes express the 5-HTT, and a B lymphocyte line 
      has been shown to express the receptor for brain-derived neurotrophic factor, 
      trkB. Since brain-derived neurotrophic factor (BDNF) is a specific growth and 
      differentiation factor for serotonergic neurons, we assessed whether BDNF is able 
      to modulate 5-HTT function in B lymphoblasts. Nerve growth factor (NGF), another 
      neurotrophin which acts via the trkA receptor, was also studied. Eight 
      immortalized B lymphoblast lines were generated and genotyped for the 5-HTTLPR. 
      After treatment with BDNF or NGF, 5-HT uptake and proliferation of the cell lines 
      were assessed. Two of the B cell lines showed a dose-dependent reduction of 5-HT 
      uptake after exposure to BDNF. Both of these cell lines were homozygous for the 
      long allele of the 5-HTTLPR. NGF did not influence 5-HT uptake or cellular 
      proliferation in any of the cell lines. Thus, BDNF but not NGF may influence 5-HT 
      uptake in some B lymphocytes. The fact that regulation of the 5-HTT was observed 
      preferentially in cells of the long/long genotype indicates that presence of a 
      short allele confers reduced regulatory capacity on the 5-HTT. In conclusion, B 
      lymphoblasts represent a practical model for functional regulation of the 5-HTT 
      by neurotrophins in serotonergic neurons.
FAU - Mossner, R
AU  - Mossner R
AD  - Department of Psychiatry, University of Wurzburg, Germany.
FAU - Daniel, S
AU  - Daniel S
FAU - Albert, D
AU  - Albert D
FAU - Heils, A
AU  - Heils A
FAU - Okladnova, O
AU  - Okladnova O
FAU - Schmitt, A
AU  - Schmitt A
FAU - Lesch, K P
AU  - Lesch KP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (SLC6A4 protein, human)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 333DO1RDJY (Serotonin)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Alleles
MH  - B-Lymphocytes/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Carrier Proteins/genetics/*physiology
MH  - Cell Line, Transformed
MH  - Gene Expression
MH  - Genotype
MH  - Herpesvirus 4, Human
MH  - Humans
MH  - Membrane Glycoproteins/genetics/*physiology
MH  - *Membrane Transport Proteins
MH  - Nerve Growth Factor/*pharmacology
MH  - *Nerve Tissue Proteins
MH  - Neuroblastoma/metabolism
MH  - Polymorphism, Genetic
MH  - Promoter Regions, Genetic
MH  - Receptor, trkA/genetics/physiology
MH  - Receptor, trkB/genetics/physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serotonin/metabolism
MH  - Serotonin Plasma Membrane Transport Proteins
MH  - Tumor Cells, Cultured
EDAT- 2000/02/17 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/17 09:00
PHST- 2000/02/17 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/17 09:00 [entrez]
AID - S0197-0186(99)00122-9 [pii]
AID - 10.1016/s0197-0186(99)00122-9 [doi]
PST - ppublish
SO  - Neurochem Int. 2000 Mar;36(3):197-202. doi: 10.1016/s0197-0186(99)00122-9.