PMID- 10677388
OWN - NLM
STAT- MEDLINE
DCOM- 20000411
LR  - 20180815
IS  - 0143-5221 (Print)
IS  - 0143-5221 (Linking)
VI  - 98
IP  - 3
DP  - 2000 Mar
TI  - Albumin stimulates p44/p42 extracellular-signal-regulated mitogen-activated 
      protein kinase in opossum kidney proximal tubular cells.
PG  - 295-301
AB  - The presence of protein in the urine of patients with renal disease is an adverse 
      prognostic feature. It has therefore been suggested that proteinuria per se may 
      be responsible for the development of renal tubulo-interstitial scarring and 
      fibrosis, and disturbances in tubular cell growth and proliferation. We have used 
      the opossum kidney proximal tubular cell line to investigate the effects of 
      albumin on cell growth. The effect of albumin on cell proliferation was 
      investigated by cell counting and measurement of [(3)H]thymidine incorporation. 
      We studied the effect of recombinant human albumin on the activity of p44/p42 
      extracellular-signal-regulated mitogen-activated protein kinase (MAP kinase ) 
      using an in vitro kinase assay, and immunoblotting with antibodies against active 
      extracellular-signal-regulated kinase (ERK). The effects of the ERK inhibitor 
      PD98059 were also examined. Recombinant human albumin was found to stimulate 
      proliferation of opossum kidney cells in a dose-dependent manner, with maximal 
      stimulation at a concentration of 1 mg/ml. In addition, recombinant human albumin 
      activated ERK in a time-dependent (maximal after 5 min) and dose-dependent 
      (maximal at 1 mg/ml) fashion. These effects on cell proliferation and ERK 
      activity were inhibited by PD98059, and were not reproduced by ovalbumin or 
      mannitol. The data therefore indicate that albumin is able to stimulate growth 
      and proliferation of proximal tubular cells that is dependent on the ERK family 
      of MAP kinases. The potential importance of this pathway in the development of 
      renal disease is discussed.
FAU - Dixon, R
AU  - Dixon R
AD  - Department of Cell Physiology and Pharmacology, Leicester University School of 
      Medicine, P.O. Box 138, Maurice Shock Medical Sciences Building, University Road, 
      Leicester LE1 9HN, UK.
FAU - Brunskill, N J
AU  - Brunskill NJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Serum Albumin)
RN  - 3OWL53L36A (Mannitol)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Humans
MH  - Kidney Tubules, Proximal/*drug effects/enzymology/pathology
MH  - Mannitol/pharmacology
MH  - Mitogen-Activated Protein Kinase 1/*metabolism
MH  - Mitogen-Activated Protein Kinases/analysis/antagonists & inhibitors/metabolism
MH  - Opossums
MH  - Ovalbumin/pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Serum Albumin/*pharmacology
MH  - Thymidine/metabolism
EDAT- 2000/03/24 09:00
MHDA- 2000/04/15 09:00
CRDT- 2000/03/24 09:00
PHST- 2000/03/24 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/03/24 09:00 [entrez]
PST - ppublish
SO  - Clin Sci (Lond). 2000 Mar;98(3):295-301.