PMID- 10677562
OWN - NLM
STAT- MEDLINE
DCOM- 20000407
LR  - 20190516
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 5
IP  - 3
DP  - 2000 Mar
TI  - Spectral karyotyping and chromosome banding studies of primary breast carcinomas 
      and their lymph node metastases.
PG  - 235-40
AB  - Three primary breast tumors and their lymph node metastases were characterized by 
      G-banding, spectral karyotyping (SKY), and fluorescence in situ hybridization 
      (FISH). In each case, the karyotypic abnormalities detected were similar in the 
      primary tumor and its matched metastasis. Two of the pairs had near-diploid 
      karyotypes with three to four chromosomal aberrations, whereas the third pair had 
      a near-pentaploid chromosome content and many marker chromosomes in the primary 
      tumor and a near-tetraploid chromosome number with almost the same marker 
      chromosomes in the metastasis. SKY and FISH confirmed the karyotypic similarities 
      between the primary tumors and their metastases and, in addition, improved the 
      identification and characterization of marker chromosomes. One of the tumor pairs 
      with near-diploid karyotypes had gain of 8q, 16q, and 17q, whereas the other had 
      gain of 1q and chromosome 8 material in the form of ring chromosomes. The third 
      pair had more complex chromosomal translocations and numerical changes resulting 
      in net gain of material from chromosomes X, 1, 2, 6, 7, 14, 16, 19, and 20, and 
      chromosome arms 8q and 11q, as well as net loss of material from chromosomes 3, 
      13, 18, 21, and 22. The present study underscores the need to combine 
      conventional chromosome banding and molecular cytogenetic techniques in the 
      cytogenetic analysis of solid tumors.
FAU - Adeyinka, A
AU  - Adeyinka A
AD  - Department of Clinical Genetics, University Hospital Lund, Sweden.
FAU - Kytola, S
AU  - Kytola S
FAU - Mertens, F
AU  - Mertens F
FAU - Pandis, N
AU  - Pandis N
FAU - Larsson, C
AU  - Larsson C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology/secondary
MH  - Chromosome Banding
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymph Nodes
MH  - Lymphatic Metastasis
EDAT- 2000/02/26 00:00
MHDA- 2000/02/26 00:01
CRDT- 2000/02/26 00:00
PHST- 2000/02/26 00:00 [pubmed]
PHST- 2000/02/26 00:01 [medline]
PHST- 2000/02/26 00:00 [entrez]
AID - 10.3892/ijmm.5.3.235 [doi]
PST - ppublish
SO  - Int J Mol Med. 2000 Mar;5(3):235-40. doi: 10.3892/ijmm.5.3.235.