PMID- 10679114
OWN - NLM
STAT- MEDLINE
DCOM- 20000323
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 5
DP  - 2000 Mar 1
TI  - Induction of chemokine secretion and enhancement of contact-dependent macrophage 
      cytotoxicity by engineered expression of granulocyte-macrophage 
      colony-stimulating factor in human colon cancer cells.
PG  - 2728-37
AB  - We investigated the role of tumor cell-derived GM-CSF in recruitment and 
      tumoricidal activation of tissue macrophages. Transfection of the murine GM-CSF 
      gene into KM12SM human colon cancer cells decreased the tumorigenicity of 
      transfected cells and nontransfected bystander colon cancer cells in nude mice. 
      Sequential tissue sections from sites injected with high GM-CSF-producing tumor 
      cells (but not from nontransfected or low GM-CSF-producing cells) demonstrated a 
      dense infiltration of polymorphonuclear cells (PMN), followed by infiltration of 
      macrophages, which correlated with expression of the macrophage-inflammatory 
      protein-1alpha and the monocyte chemoattractant protein-1 (MCP-1) in mouse PMN 
      and macrophages. GM-CSF-producing KM12SM cells were highly sensitive to lysis by 
      mouse macrophages and also increased macrophage-mediated lysis of bystander 
      nontransfected KM12SM cells. The incubation of macrophages with GM-CSF induced 
      expression of the CD11b surface adhesion molecule, which was associated with 
      increased attachment to tumor cells. All KM12SM cells were sensitive to 
      macrophage-mediated lysis in the presence of rGM-CSF and recombinant MCP-1. 
      Collectively, the results demonstrate that tumor cell-derived GM-CSF stimulates 
      PMN and macrophages to secrete macrophage-inflammatory protein-1alpha and MCP-1, 
      which triggers recruitment of mononuclear cells, induces expression of adhesion 
      molecules on macrophages, and enhances contact-dependent cytolysis of tumor 
      cells.
FAU - Shinohara, H
AU  - Shinohara H
AD  - Department of Cancer Biology, University of Texas M. D. Anderson Cancer Center, 
      Houston, TX 77030, USA.
FAU - Yano, S
AU  - Yano S
FAU - Bucana, C D
AU  - Bucana CD
FAU - Fidler, I J
AU  - Fidler IJ
LA  - eng
GR  - CA16672/CA/NCI NIH HHS/United States
GR  - R35-CA42107/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Recombinant Proteins)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Cell Communication/genetics/*immunology
MH  - Cell Movement/genetics/immunology
MH  - Chemokine CCL2/biosynthesis/genetics/metabolism
MH  - Chemokine CCL4
MH  - Chemokines/biosynthesis/genetics/*metabolism
MH  - Coculture Techniques
MH  - Colonic Neoplasms/genetics/*immunology/*metabolism/prevention & control
MH  - Cytotoxicity, Immunologic/genetics/*immunology
MH  - Gene Expression Regulation, Neoplastic/immunology
MH  - Granulocyte-Macrophage Colony-Stimulating 
      Factor/*biosynthesis/genetics/*immunology/pharmacology
MH  - HT29 Cells
MH  - Humans
MH  - Injections, Subcutaneous
MH  - Macrophage Inflammatory Proteins/biosynthesis/genetics
MH  - Macrophage-1 Antigen/biosynthesis
MH  - Macrophages/*immunology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Neutrophils/immunology
MH  - Protein Engineering
MH  - Recombinant Proteins
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2000/02/29 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/02/29 09:00
PHST- 2000/02/29 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/02/29 09:00 [entrez]
AID - ji_v164n5p2728 [pii]
AID - 10.4049/jimmunol.164.5.2728 [doi]
PST - ppublish
SO  - J Immunol. 2000 Mar 1;164(5):2728-37. doi: 10.4049/jimmunol.164.5.2728.