PMID- 10680749
OWN - NLM
STAT- MEDLINE
DCOM- 20000306
LR  - 20190921
IS  - 0891-6934 (Print)
IS  - 0891-6934 (Linking)
VI  - 31
IP  - 2
DP  - 1999 Oct
TI  - Anti-Beta 2-glycoprotein I antibody isotype and IgG subclass in antiphospholipid 
      syndrome patients.
PG  - 109-16
AB  - Beta 2-glycoprotein I (beta2-GPI) is an antigenic target recognised by 
      antiphospholipid antibodies found in association with the antiphospholipid 
      syndrome (APS). In this study, the prevalence of Immunoglobulin M (IgM) and IgA 
      anti-beta2-GPI antibodies was examined in APS patients and compared with IgG 
      antibodies. In addition the value of measuring antibody isotypes and IgG subclass 
      was investigated in the laboratory diagnosis of APS. A solid phase enzyme linked 
      immunosorbent assay was established to measure IgG, IgM and IgA and IgG subclass 
      antibodies to beta2-GPI in patients with APS and a variety of other thrombotic 
      and non-thrombotic disorders. Raised levels of IgM anti-beta2-GPI antibodies were 
      observed in 65% of patients with APS, 21% with systemic lupus erythematosus 
      (SLE), 23% with rheumatoid factor, 4% with stroke, 5% carotid artery stenosis 
      (CAS), 17% with a biological false positive serology for syphilis, 43% with 
      infectious mononucleosis (IM) and 27% with human immunodeficiency virus (HIV). 
      The median value for IgM antibodies to beta2-GPI for all these groups ranged from 
      2 to 7 arbitrary units (AU). Elevated levels of IgA antibodies to beta2-GPI were 
      found in patients with APS (47%), SLE (13%), rheumatoid factor (26%), CAS (48%), 
      stroke (25%), VDRL false positive serology for syphilis (33%), IM (47%) and HIV 
      (7%). The median value of IgA antibodies to beta2-GPI in all of these groups 
      ranged from 2 to 4 AU. Conversely the median value for IgG anti-beta2-GPI in APS 
      patients was 112 AU compared to 1-4 AU in the other conditions examined. The 
      presence of IgM and IgA antibodies to beta2-GPI was much less specific and 
      sensitive for APS than IgG, with raised levels of these isotypes seen in a 
      variety of thrombotic and non-thrombotic disorders. Elevated levels of IgG1, 
      IgG2, IgG3 and IgG4 antibodies to beta2-GPI were detected in APS patients. While 
      all four IgG anti-beta2-GPI antibody subclasses were represented in APS patients 
      there appeared to be a significant overall skewing towards to the IgG2 subclass.
FAU - Guerin, J
AU  - Guerin J
AD  - Department of Immunology, St. James's Hospital, Dublin, Ireland.
FAU - Casey, E
AU  - Casey E
FAU - Feighery, C
AU  - Feighery C
FAU - Jackson, J
AU  - Jackson J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (Anticoagulants)
RN  - 0 (Biomarkers)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Immunoglobulin M)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Antibody Specificity
MH  - Anticoagulants/blood/immunology
MH  - Antiphospholipid Syndrome/blood/*immunology
MH  - Biomarkers/blood
MH  - Evaluation Studies as Topic
MH  - Glycoproteins/blood/*immunology
MH  - Humans
MH  - Immunoglobulin A/blood
MH  - Immunoglobulin G/blood
MH  - Immunoglobulin Isotypes/*blood
MH  - Immunoglobulin M/blood
MH  - Thrombosis/blood/immunology
MH  - beta 2-Glycoprotein I
EDAT- 2000/02/19 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/19 09:00
PHST- 2000/02/19 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/19 09:00 [entrez]
AID - 10.3109/08916939908994054 [doi]
PST - ppublish
SO  - Autoimmunity. 1999 Oct;31(2):109-16. doi: 10.3109/08916939908994054.