PMID- 10683151
OWN - NLM
STAT- MEDLINE
DCOM- 20000531
LR  - 20220318
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 113 ( Pt 6)
DP  - 2000 Mar
TI  - Evidence for the coincident initiation of homolog pairing and synapsis during the 
      telomere-clustering (bouquet) stage of meiotic prophase.
PG  - 1033-42
AB  - To improve knowledge of the prerequisites for meiotic chromosome segregation in 
      higher eukaryotes, we analyzed the spatial distribution of a pair of homologs 
      before and during early meiotic prophase. Three-dimensional images of 
      fluorescence in situ hybridization (FISH) were used to localize a single pair of 
      homologs in diploid nuclei of a chromosome-addition line of oat, oat-maize9b. The 
      system provided a robust assay for pairing based on cytological colocalization of 
      FISH signals. Using a triple labeling scheme for simultaneous imaging of 
      chromatin, telomeres and the homolog pair, we determined the timing of pairing in 
      relation to the onset of three sequential hallmarks of early meiotic prophase: 
      chromatin condensation (the leptotene stage), meiotic telomere clustering (the 
      bouquet stage) and the initiation of synapsis (the zygotene stage). We found that 
      the two homologs were mostly unpaired up through middle leptotene, at which point 
      their spherical cloud-like domains began to transform into elongated and 
      stretched-out domains. At late leptotene, the homologs had completely reorganized 
      into long extended fibers, and the beginning of the bouquet stage was 
      conspicuously marked by the de novo clustering of telomeres at the nuclear 
      periphery. The homologs paired and synapsed during the bouquet stage, consistent 
      with the timing of pairing observed for several oat 5S rDNA loci. In summary, 
      results from analysis of more than 100 intact nuclei lead us to conclude that 
      pairing and synapsis of homologous chromosomes are largely coincident processes, 
      ruling out a role for premeiotic pairing in this system. These findings suggest 
      that the genome-wide remodeling of chromatin and telomere-mediated nuclear 
      reorganization are prerequisite steps to the DNA sequence-based homology-search 
      process in higher eukaryotes.
FAU - Bass, H W
AU  - Bass HW
AD  - Department of Molecular, University of California, Berkeley, Berkeley, CA 94720, 
      USA. bass@bio.fsu.edu
FAU - Riera-Lizarazu, O
AU  - Riera-Lizarazu O
FAU - Ananiev, E V
AU  - Ananiev EV
FAU - Bordoli, S J
AU  - Bordoli SJ
FAU - Rines, H W
AU  - Rines HW
FAU - Phillips, R L
AU  - Phillips RL
FAU - Sedat, J W
AU  - Sedat JW
FAU - Agard, D A
AU  - Agard DA
FAU - Cande, W Z
AU  - Cande WZ
LA  - eng
GR  - R01-GM-25101-16/GM/NIGMS NIH HHS/United States
GR  - R01-GM-48547/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
SB  - IM
MH  - Avena
MH  - Base Pairing
MH  - In Situ Hybridization, Fluorescence
MH  - *Meiosis
MH  - *Telomere/ultrastructure
MH  - Zea mays
EDAT- 2000/02/22 09:00
MHDA- 2000/06/03 09:00
CRDT- 2000/02/22 09:00
PHST- 2000/02/22 09:00 [pubmed]
PHST- 2000/06/03 09:00 [medline]
PHST- 2000/02/22 09:00 [entrez]
AID - 10.1242/jcs.113.6.1033 [doi]
PST - ppublish
SO  - J Cell Sci. 2000 Mar;113 ( Pt 6):1033-42. doi: 10.1242/jcs.113.6.1033.