PMID- 10683275
OWN - NLM
STAT- MEDLINE
DCOM- 20000323
LR  - 20220223
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 161
IP  - 1
DP  - 2000 Jan
TI  - Experimental gliosarcoma induces chemokine receptor expression in rat brain.
PG  - 85-95
AB  - Macrophage/microglial infiltration is a characteristic feature of brain tumors. 
      The functional role(s) of these cells is complex and could include both trophic 
      and suppressive effects on tumors. Information has recently emerged about the 
      molecular signals that regulate the accumulation and function of monocytes in 
      pathological disorders. Recent data indicate that the chemokine, monocyte 
      chemoattractant protein-1 (MCP-1), a potent monocyte activating and chemotactic 
      factor, is a primary regulator of the macrophage response in brain tumors. We 
      hypothesized that if MCP-1 regulates macrophage/microglial infiltration, then 
      expression of the specific MCP-1 receptor, CCR2, will be induced in peritumoral 
      tissue and/or within brain tumors. Identification of a specific receptor that is 
      preferentially expressed in brain tumors could be important both in terms of 
      tumor biology and as a potential therapeutic target. We used an established 
      experimental gliosarcoma model, induced by intracranial transplantation of 
      cultured 9L cells into adult rat brain, to test this hypothesis. RT-PCR analysis 
      showed high levels of both MCP-1 and CCR2 mRNA and Western blot analysis 
      demonstrated increased CCR2 protein in tumor extracts. Immunocytochemistry showed 
      CCR2 immunoreactive microglia in peritumoral tissue and, unexpectedly, that 
      intrinsic tumor cells, rather than monocytes, were the predominant source of 
      CCR2. These results demonstrate that CCR2 expression is markedly upregulated in 
      this brain tumor model.
CI  - Copyright 2000 Academic Press.
FAU - Galasso, J M
AU  - Galasso JM
AD  - Neuroscience Program, University of Michigan, Ann Arbor, Michigan, 48109-0646, 
      USA.
FAU - Stegman, L D
AU  - Stegman LD
FAU - Blaivas, M
AU  - Blaivas M
FAU - Harrison, J K
AU  - Harrison JK
FAU - Ross, B D
AU  - Ross BD
FAU - Silverstein, F S
AU  - Silverstein FS
LA  - eng
GR  - NS31054/NS/NINDS NIH HHS/United States
GR  - NS35059/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Autoantigens)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Ccr2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA Primers)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Cytokine)
SB  - IM
MH  - Animals
MH  - Autoantigens/analysis/*genetics
MH  - Blotting, Western
MH  - Brain Chemistry/genetics/immunology
MH  - Brain Neoplasms/diagnosis/*immunology/physiopathology
MH  - *Chemokine CCL2
MH  - DNA Primers
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Gliosarcoma/diagnosis/*immunology/physiopathology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/immunology
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Microglia/physiology
MH  - Neoplasm Transplantation
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred F344
MH  - Receptors, CCR2
MH  - *Receptors, Chemokine
MH  - Receptors, Cytokine/analysis/*genetics
EDAT- 2000/02/23 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/02/23 09:00
PHST- 2000/02/23 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/02/23 09:00 [entrez]
AID - S0014-4886(99)97249-7 [pii]
AID - 10.1006/exnr.1999.7249 [doi]
PST - ppublish
SO  - Exp Neurol. 2000 Jan;161(1):85-95. doi: 10.1006/exnr.1999.7249.