PMID- 10683319
OWN - NLM
STAT- MEDLINE
DCOM- 20000915
LR  - 20131121
IS  - 1522-4724 (Print)
IS  - 1522-4724 (Linking)
VI  - 3
IP  - 1
DP  - 2000 Jan
TI  - Induction and secretion of the chemokines interleukin-8 and monocyte chemotactic 
      protein-1 in human immature leukemia cell lines.
PG  - 60-5
AB  - We investigated expression and secretion of the chemokines interleukin-8 (IL-8) 
      and monocyte chemotactic protein-1 (MCP-1) in human myeloid cell lines. 
      Quantitative determination by ELISA revealed a significant constitutive 
      production of both chemokines in the cell lines HL-60 and NB-4 (>1000 pg/ml IL-8 
      and >400 pg/ml MCP-1 per million cells), while in the cell lines EOL-1, KASUMI-1 
      and KG-1 only 10-100 pg/ml IL-8 and MCP-1 were detected. Tetradecanoyl phorbol 
      acetate (TPA) strongly increased the IL-8 and MCP-1 amounts in the culture 
      supernatants of all five cell lines. The TPA-induced NB-4 produced the largest 
      amounts of both chemokines (>40,000 pg/ml). The strongest induction was seen in 
      EOL-1 (>100-fold increase). Besides TPA, tumor necrosis factor-alpha (TNF alpha) 
      also distinctively enhanced IL-8 and MCP-1 production. The calcium ionophore 
      A-23187 and thapsigargin, an inhibitor of the Ca(2+)-ATPase, differentially 
      induced IL-8 and MCP-1 secretion in the cell lines investigated, suggesting that, 
      at least in some cell lines, intracellular free Ca(2+) might be important for 
      chemokine secretion. Dexamethasone significantly prevented the IL-8 and MCP-1 
      production of stimulated cells, emphasizing the potent anti-inflammatory property 
      of glucocorticoids. Similarly, the protein kinase inhibitor staurosporine clearly 
      decreased the TPA-induced chemokine secretion in NB-4 cells, indicating the 
      involvement of protein kinases in the signal transduction pathway which leads to 
      enhanced chemokine secretion.
CI  - Copyright 2000 Academic Press.
FAU - Steube, K G
AU  - Steube KG
AD  - Department of Human and Animal Cell Cultures, DSMZ-German Collection of 
      Microorganisms and Cell Cultures, Braunschweig, Germany. kst@dsmz.de
FAU - Meyer, C
AU  - Meyer C
FAU - Drexler, H G
AU  - Drexler HG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol Res Commun
JT  - Molecular cell biology research communications : MCBRC
JID - 100889076
RN  - 0 (Carcinogens)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glucocorticoids)
RN  - 0 (Interleukin-8)
RN  - 0 (Ionophores)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - H88EPA0A3N (Staurosporine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Calcimycin/pharmacology
MH  - Carcinogens
MH  - Chemokine CCL2/antagonists & inhibitors/*biosynthesis/genetics
MH  - Chemokines/biosynthesis/genetics
MH  - Dexamethasone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glucocorticoids/pharmacology
MH  - HL-60 Cells
MH  - Humans
MH  - Interleukin-8/antagonists & inhibitors/*biosynthesis/genetics
MH  - Ionophores/pharmacology
MH  - Leukemia/*metabolism
MH  - Protein Biosynthesis/drug effects
MH  - Staurosporine/pharmacology
MH  - Tetradecanoylphorbol Acetate
MH  - Thapsigargin/pharmacology
MH  - Transcription, Genetic/drug effects
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2000/02/23 09:00
MHDA- 2000/09/23 11:01
CRDT- 2000/02/23 09:00
PHST- 2000/02/23 09:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/02/23 09:00 [entrez]
AID - S1522472400901909 [pii]
AID - 10.1006/mcbr.2000.0190 [doi]
PST - ppublish
SO  - Mol Cell Biol Res Commun. 2000 Jan;3(1):60-5. doi: 10.1006/mcbr.2000.0190.