PMID- 10683440
OWN - NLM
STAT- MEDLINE
DCOM- 20000410
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 468
IP  - 1
DP  - 2000 Feb 18
TI  - ERK activation upon hypoxia: involvement in HIF-1 activation.
PG  - 53-8
AB  - Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by 
      hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this 
      report, we investigated the activation of extracellular regulated kinases (ERK) 
      in hypoxia and their involvement in HIF-1 activation. We demonstrated that in 
      human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated 
      during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed 
      for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase 
      assay and Western blot experiments, we showed that HIF-1alpha is phosphorylated 
      in hypoxia by an ERK-dependent pathway. These results evidence the role of 
      mitogen-activated protein kinase in the transcriptional response to hypoxia.
FAU - Minet, E
AU  - Minet E
AD  - Laboratoire de Biochimie et Biologie Cellulaire, Facultes Universitaires de la 
      Paix, 61 rue de Bruxelles, 5000, Namur, Belgium. emmanuel.minet@fundp.ac.be
FAU - Arnould, T
AU  - Arnould T
FAU - Michel, G
AU  - Michel G
FAU - Roland, I
AU  - Roland I
FAU - Mottet, D
AU  - Mottet D
FAU - Raes, M
AU  - Raes M
FAU - Remacle, J
AU  - Remacle J
FAU - Michiels, C
AU  - Michiels C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Blotting, Western
MH  - Cell Hypoxia/*physiology
MH  - Cell Line
MH  - Cell Nucleus/enzymology
MH  - DNA-Binding Proteins/*metabolism
MH  - Endothelium, Vascular/cytology/enzymology
MH  - Enzyme Activation/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Microcirculation/cytology/enzymology
MH  - Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/*metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Structure, Tertiary
MH  - Transcription Factors/metabolism
MH  - Tyrosine/metabolism
EDAT- 2000/02/23 09:00
MHDA- 2000/04/15 09:00
CRDT- 2000/02/23 09:00
PHST- 2000/02/23 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/02/23 09:00 [entrez]
AID - S0014-5793(00)01181-9 [pii]
AID - 10.1016/s0014-5793(00)01181-9 [doi]
PST - ppublish
SO  - FEBS Lett. 2000 Feb 18;468(1):53-8. doi: 10.1016/s0014-5793(00)01181-9.