PMID- 10683829
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Jan 17
TI  - Pro-apoptotic effects of tau mutations in chromosome 17 frontotemporal dementia 
      and parkinsonism.
PG  - 57-60
AB  - It was recently discovered that mutations of tau cause hereditary frontotemporal 
      dementia and parkinsonism linked to chromosome 17 (FTDP-17). Here we report that 
      cultured SH-SY5Y human neuroblastoma cells transfected with mutated tau genes are 
      more vulnerable to apoptotic stimulus. Two kinds of mutations of tau causing 
      FTDP-17 were examined in the present study: one was in exon 10 (N279K) and the 
      other was in exon 12 (V337M). SH-SY5Y cells transfected with either mutated tau 
      were more vulnerable to serum withdrawal, whereas cells transfected with the 
      wild-type tau or vector alone showed no significant change in apoptotic 
      vulnerability. The increase in the intracellular calcium concentration by the 
      serum withdrawal was significantly greater in the SH-SY5Y cells transfected with 
      mutated tau genes than in cells transfected with the wild-type tau or vector 
      alone. These results suggest that mutations of tau might cause FTDP-17 by these 
      pro-apoptotic functions by disrupting the intracellular calcium homeostasis.
FAU - Furukawa, K
AU  - Furukawa K
AD  - Geriatric Research Education and Clinical Center, Veterans Affairs Puget Sound 
      Health Care System, Seattle, WA 98108, USA.
FAU - D'Souza, I
AU  - D'Souza I
FAU - Crudder, C H
AU  - Crudder CH
FAU - Onodera, H
AU  - Onodera H
FAU - Itoyama, Y
AU  - Itoyama Y
FAU - Poorkaj, P
AU  - Poorkaj P
FAU - Bird, T D
AU  - Bird TD
FAU - Schellenberg, G D
AU  - Schellenberg GD
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (tau Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Blotting, Western
MH  - Cell Line
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - DNA/genetics
MH  - Dementia/*genetics/pathology
MH  - Exons/genetics
MH  - Frontal Lobe/pathology/*physiology
MH  - Homeostasis
MH  - Humans
MH  - Mutation/*genetics/physiology
MH  - Parkinson Disease/*genetics/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Temporal Lobe/pathology/*physiology
MH  - Transfection/genetics
MH  - Tumor Cells, Cultured
MH  - tau Proteins/*genetics
EDAT- 2000/02/23 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/02/23 09:00
PHST- 2000/02/23 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/23 09:00 [entrez]
AID - 10.1097/00001756-200001170-00011 [doi]
PST - ppublish
SO  - Neuroreport. 2000 Jan 17;11(1):57-60. doi: 10.1097/00001756-200001170-00011.