PMID- 10685080
OWN - NLM
STAT- MEDLINE
DCOM- 20000405
LR  - 20171116
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 39
IP  - 3
DP  - 2000 Mar 1
TI  - Fetal nucleated erythrocyte recovery: fluorescence activated cell sorting-based 
      positive selection using anti-gamma globin versus magnetic activated cell sorting 
      using anti-CD45 depletion and anti-gamma globin positive selection.
PG  - 224-30
AB  - BACKGROUND: Fluorescence activated cell sorting (FACS)-based anti-gamma (gamma) 
      positive selection and magnetic activated cell sorting (MACS)-based anti-CD45 
      depletion followed by anti-gamma positive staining have been two of the most 
      frequently used methods to isolate fetal cells from maternal blood. To date, 
      there has been no direct comparison of fetal cell recovery by these two methods. 
      This study was designed to address this issue. METHODS: Fluorescence in situ 
      hybridization (FISH) was performed on nucleated anti-gamma positive cells using X 
      and Y probes. Twenty-four maternal blood samples were obtained immediately after 
      elective termination of pregnancy to ensure a detectable number of fetal cells. 
      RESULTS: The yield and purity of fetal nucleated erythrocytes (FNRBCs) was 
      statistically higher in FACS sorted samples (P < 0.01). The specificity of 
      staining for FNRBCs was statistically higher in MACS sorted samples (P < 0.01). 
      CONCLUSIONS: The data from this study demonstrate that both techniques have 
      benefits and limitations. FACS has the advantage of having higher yield, higher 
      purity, higher FISH efficiency and ease in microscope analysis, and MACS has the 
      advantage of having higher specificity and less cell loss during FISH.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Wang, J Y
AU  - Wang JY
AD  - Division of Genetics, Department of Pediatrics, New England Medical Center, Tufts 
      University School of Medicine, Boston, Massachusetts 02111, USA.
FAU - Zhen, D K
AU  - Zhen DK
FAU - Falco, V M
AU  - Falco VM
FAU - Farina, A
AU  - Farina A
FAU - Zheng, Y L
AU  - Zheng YL
FAU - Delli-Bovi, L C
AU  - Delli-Bovi LC
FAU - Bianchi, D W
AU  - Bianchi DW
LA  - eng
GR  - N01-HD-43204/HD/NICHD NIH HHS/United States
GR  - P01-HD-18658/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Antigens, CD)
RN  - 9004-22-2 (Globins)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Cell Nucleus/ultrastructure
MH  - Cell Separation/methods
MH  - Embryo, Mammalian
MH  - Erythrocytes/*cytology/ultrastructure
MH  - Female
MH  - Fetal Blood/cytology
MH  - Fetus
MH  - Flow Cytometry/*methods
MH  - Gestational Age
MH  - Globins/*analysis
MH  - Humans
MH  - Immunomagnetic Separation/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocyte Common Antigens/analysis
MH  - Pregnancy
EDAT- 2000/02/24 00:00
MHDA- 2000/02/24 00:01
CRDT- 2000/02/24 00:00
PHST- 2000/02/24 00:00 [pubmed]
PHST- 2000/02/24 00:01 [medline]
PHST- 2000/02/24 00:00 [entrez]
AID - 10.1002/(SICI)1097-0320(20000301)39:3<224::AID-CYTO8>3.0.CO;2-J [pii]
PST - ppublish
SO  - Cytometry. 2000 Mar 1;39(3):224-30.