PMID- 10686079
OWN - NLM
STAT- MEDLINE
DCOM- 20000329
LR  - 20081121
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 161
IP  - 2
DP  - 2000 Feb
TI  - Contraction-induced muscle fiber damage is increased in soleus muscle of 
      streptozotocin-diabetic rats and is associated with elevated expression of 
      brain-derived neurotrophic factor mRNA in muscle fibers and activated satellite 
      cells.
PG  - 597-608
AB  - The expression of brain-derived neurotrophic factor (BDNF) is elevated in the 
      soleus muscle of streptozotocin-diabetic rats. To determine whether this 
      diabetes-induced elevation was associated with or enhanced by muscle activity we 
      have induced high-intensity muscle contraction by electrically stimulating the 
      sciatic nerve. In 6-week diabetic rats, intense contraction of the soleus muscle 
      resulted in a two- to four-fold elevation of BDNF mRNA and increased plasma 
      levels of creatine kinase that were associated with severe focal muscle fiber 
      damage and concomitant satellite cell activation. Focal muscle fiber damage and 
      concomitant satellite cell activation were also observed in the soleus muscle of 
      nonstimulated diabetic rats, but to a much lesser extent. No effects of muscle 
      contraction, i.e., experimentally induced or during normal daily activity, on 
      muscle fiber structure or BDNF mRNA expression were seen in diabetic extensor 
      digitorum longus (EDL) muscle. Using a nonradioactive in situ hybridization 
      technique for electron microscopy, the elevated expression of BDNF mRNA in the 
      diabetic soleus muscle was localized within muscle fibers as well as activated 
      satellite cells. This study shows that diabetic soleus muscle, in contrast to 
      diabetic EDL and to soleus and EDL muscle of normal animals, is highly 
      susceptible to contraction-induced damage. Intense contraction and the associated 
      muscle fiber damage in the diabetic soleus muscle result in an upregulation of 
      BDNF mRNA in muscle fibers and activated satellite cells, which may be involved 
      in the restoration and/or maintenance of nerve/muscle integrity.
CI  - Copyright 2000 Academic Press.
FAU - Copray, S
AU  - Copray S
AD  - Department of Medical Physiology, University of Groningen, Groningen, 9712 KZ, 
      The Netherlands.
FAU - Liem, R
AU  - Liem R
FAU - Brouwer, N
AU  - Brouwer N
FAU - Greenhaff, P
AU  - Greenhaff P
FAU - Habens, F
AU  - Habens F
FAU - Fernyhough, P
AU  - Fernyhough P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Diabetes Mellitus, Experimental/genetics/pathology/*physiopathology
MH  - Electric Stimulation
MH  - Gene Expression Regulation
MH  - Kinetics
MH  - Male
MH  - Muscle Contraction/physiology
MH  - Muscle Fibers, Skeletal/*metabolism/pathology
MH  - Muscle, Skeletal/metabolism/pathology/*physiopathology
MH  - Neuroglia/*metabolism/pathology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Reference Values
MH  - Sciatic Nerve/physiopathology
MH  - *Transcription, Genetic
EDAT- 2000/02/25 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/02/25 09:00
PHST- 2000/02/25 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/02/25 09:00 [entrez]
AID - S0014-4886(99)97306-5 [pii]
AID - 10.1006/exnr.1999.7306 [doi]
PST - ppublish
SO  - Exp Neurol. 2000 Feb;161(2):597-608. doi: 10.1006/exnr.1999.7306.