PMID- 10686086
OWN - NLM
STAT- MEDLINE
DCOM- 20000329
LR  - 20081121
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 161
IP  - 2
DP  - 2000 Feb
TI  - Growth factors in combination, but not individually, rescue rd mouse 
      photoreceptors in organ culture.
PG  - 676-85
AB  - The rd mouse retina is an animal model for human retinal dystrophy in which the 
      rod photoreceptors undergo apoptosis during the first 4 weeks in vivo or in organ 
      culture. We have examined the effect of different families of trophic factors on 
      the survival of rd mouse photoreceptors in organ culture. Retinas were harvested 
      from rd mice at postnatal day 2 and grown in organ culture for 27 days in vitro 
      (DIV) in DMEM with 10% fetal calf serum. Ciliary neurotrophic factor (CNTF), 
      brain-derived neurotrophic factor (BDNF), fibroblast growth factor-2 (FGF2), 
      glial cell line-derived neurotrophic factor (GDNF), neurturin, and persephon were 
      added individually or in combination to the medium at a dose of 50 ng/ml or less. 
      CNTF + BDNF in combination resulted in photoreceptor survival comparable to 
      wild-type retinas after 27 DIV. CNTF + FGF2 or CNTF + GDNF produced a partial 
      prevention of photoreceptor death. Photoreceptor degeneration was not blocked by 
      any of the trophic factors added individually. A significant increase in 
      photoreceptor survival was seen with forskolin added to CNTF, but not to BDNF, 
      FGF2, or GDNF. These results demonstrate that trophic factors promote 
      photoreceptor survival through a synergistic interaction. Increased understanding 
      of receptor interactions and signaling pathways may lead to a potential 
      therapeutic role for combinatorial trophic factors in treatment of photoreceptor 
      dystrophies.
CI  - Copyright 2000 Academic Press.
FAU - Ogilvie, J M
AU  - Ogilvie JM
AD  - Fay and Carl Simons Center for Biology of Hearing and Deafness, Central Institute 
      for the Deaf, St. Louis, Missouri, 63110, USA.
FAU - Speck, J D
AU  - Speck JD
FAU - Lett, J M
AU  - Lett JM
LA  - eng
GR  - K17 NS0156/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (GDNF protein, human)
RN  - 0 (Gdnf protein, mouse)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Growth Substances)
RN  - 0 (NRTN protein, human)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurturin)
RN  - 0 (Nrtn protein, mouse)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Survival/drug effects
MH  - Ciliary Neurotrophic Factor/pharmacology
MH  - Disease Models, Animal
MH  - Drug Interactions
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Growth Substances/*pharmacology
MH  - Homozygote
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/pharmacology
MH  - Neurturin
MH  - Organ Culture Techniques
MH  - Photoreceptor Cells, Vertebrate/cytology/drug effects/*pathology
MH  - Retina/pathology
MH  - Retinal Degeneration/genetics/*pathology
MH  - Time Factors
EDAT- 2000/02/25 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/02/25 09:00
PHST- 2000/02/25 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/02/25 09:00 [entrez]
AID - S0014-4886(99)97291-6 [pii]
AID - 10.1006/exnr.1999.7291 [doi]
PST - ppublish
SO  - Exp Neurol. 2000 Feb;161(2):676-85. doi: 10.1006/exnr.1999.7291.