PMID- 10688910
OWN - NLM
STAT- MEDLINE
DCOM- 20000411
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 97
IP  - 5
DP  - 2000 Feb 29
TI  - Differential regulation by multiple promoters of the gene encoding the 
      neuron-restrictive silencer factor.
PG  - 2291-6
AB  - NRSF/REST is a protein that silences transcription of a number of genes that 
      contain a DNA element called the neuron-restrictive silencer element (NRSE). 
      During embryogenesis, REST is expressed ubiquitously in nonneural cells, but is 
      down-regulated during differentiation of neural progenitors into neurons. REST is 
      also up-regulated in adult neurons by activity, suggesting a possible role for 
      the protein in synaptic plasticity. To understand mechanisms that control 
      expression of REST, we identified and characterized the promoter region of the 
      mouse REST gene (mREST). A 4.5-kb DNA segment containing three exons (A, B, and 
      C) that correspond to alternatively spliced 5' untranslated regions (5'UTRs) was 
      isolated and its DNA sequence was determined. Reverse transcription-PCR analyses 
      of fibroblasts, astrocytes, and neural progenitors identified variants in which 
      these exons were spliced to exon D, suggesting that exons A, B, and C may each 
      have a promoter. Consistent with this hypothesis, primer extension and in vitro 
      transcription experiments revealed clusters of RNA transcription initiation sites 
      upstream of exons A, B, and C. Tests of REST/luciferase reporter constructs in 
      Neuro2A and NIH 3T3 cells revealed promoters upstream of exons A and B that were 
      active in both cell lines, and a promoter upstream of exon C that was weakly 
      active only in NIH 3T3 cells. Six enhancer and two repressor regions were found 
      to overlap each of the three promoters, and some of these were found to be cell 
      type-specific. Combinatorial arrangements of these promoters with enhancer and 
      repressor regions may allow modulation of REST expression in particular contexts.
FAU - Koenigsberger, C
AU  - Koenigsberger C
AD  - Department of Neurobiology, The Scripps Research Institute, 10550 North Torrey 
      Pines Road, La Jolla, CA 92037, USA.
FAU - Chicca, J J 2nd
AU  - Chicca JJ 2nd
FAU - Amoureux, M C
AU  - Amoureux MC
FAU - Edelman, G M
AU  - Edelman GM
FAU - Jones, F S
AU  - Jones FS
LA  - eng
SI  - GENBANK/AF220154
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - 3T3 Cells
MH  - 5' Untranslated Regions
MH  - Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary
MH  - Enhancer Elements, Genetic
MH  - Exons
MH  - *Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger
MH  - Repressor Proteins/*genetics
MH  - *Transcription Factors
MH  - Tumor Cells, Cultured
PMC - PMC15794
EDAT- 2000/02/26 09:00
MHDA- 2000/04/15 09:00
PMCR- 2000/08/29
CRDT- 2000/02/26 09:00
PHST- 2000/02/26 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/02/26 09:00 [entrez]
PHST- 2000/08/29 00:00 [pmc-release]
AID - 050578797 [pii]
AID - 5787 [pii]
AID - 10.1073/pnas.050578797 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2291-6. doi: 10.1073/pnas.050578797.