PMID- 10692034
OWN - NLM
STAT- MEDLINE
DCOM- 20000315
LR  - 20240322
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 99
IP  - 2
DP  - 2000 Feb
TI  - Inhibition of immunoglobulin E response to Japanese cedar pollen allergen (Cry j 
      1) in mice by DNA immunization: different outcomes dependent on the plasmid DNA 
      inoculation method.
PG  - 179-86
AB  - To develop a new immunotherapy for Japanese cedar (Cryptomeria japonica; CJ) 
      pollinosis, we evaluated the use of DNA immunization by inoculating mice with 
      plasmid DNA encoding Cry j 1 as a CJ pollen major allergen (pCACJ1). Repeated 
      intramuscular (i.m.) inoculation of BALB/c mice with pCACJ1 produced anti-Cry j 1 
      antibody responses, which were predominately of the immunoglobulin G2a (IgG2a) 
      type. Furthermore, this inoculation suppressed immunoglobulin E (IgE) and IgG1 
      antibody responses to subsequent alum-precipitated Cry j 1 injections. Splenic T 
      cells isolated from mice inoculated with pCACJ1 i.m. secreted interferon-gamma 
      (IFN-gamma), but not interleukin (IL)-4, in vitro upon stimulation with Cry j 1 
      as well as with p277-288, a peptide corresponding to the T-cell epitope of Cry j 
      1. In contrast, inoculation of BALB/c mice with pCACJ1 by gene gun injection 
      caused response predominantly of the IgG1 type, and enhanced production of 
      anti-Cry j 1 IgE antibodies to subsequent alum-precipitated Cry j 1 injections. 
      Splenic T cells isolated from pCACJ1-innoculated mice by gene gun injection 
      secreted both IFN-gamma and IL-4 in vitro, upon stimulation with Cry j 1 as well 
      as with p277-288. These findings suggest that i.m. inoculation with pCACJ1 
      effectively elicits Cry j 1-specific T helper 1 (Th1)-type immune responses, 
      resulting in inhibition of the IgE response to Cry j 1.
FAU - Toda, M
AU  - Toda M
AD  - Department of Immunology, AIDS Research Center and Infectious Disease 
      Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan.
FAU - Sato, H
AU  - Sato H
FAU - Takebe, Y
AU  - Takebe Y
FAU - Taniguchi, Y
AU  - Taniguchi Y
FAU - Saito, S
AU  - Saito S
FAU - Inouye, S
AU  - Inouye S
FAU - Takemori, T
AU  - Takemori T
FAU - Sakaguchi, M
AU  - Sakaguchi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Cry j I protein, Cryptomeria japonica)
RN  - 0 (Plant Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Antigens, Plant
MH  - Biolistics
MH  - Cell Culture Techniques
MH  - Desensitization, Immunologic/*methods
MH  - Female
MH  - Immune Tolerance
MH  - Immunization/methods
MH  - Immunoglobulin E/*biosynthesis
MH  - Injections, Intramuscular
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plant Proteins/*immunology
MH  - Pollen/*immunology
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
MH  - Vaccines, DNA/*administration & dosage/therapeutic use
PMC - PMC2327145
EDAT- 2000/02/26 09:00
MHDA- 2000/03/18 09:00
PMCR- 2001/02/01
CRDT- 2000/02/26 09:00
PHST- 2000/02/26 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/26 09:00 [entrez]
PHST- 2001/02/01 00:00 [pmc-release]
AID - imm935 [pii]
AID - 10.1046/j.1365-2567.2000.00935.x [doi]
PST - ppublish
SO  - Immunology. 2000 Feb;99(2):179-86. doi: 10.1046/j.1365-2567.2000.00935.x.