PMID- 10692159
OWN - NLM
STAT- MEDLINE
DCOM- 20000418
LR  - 20190725
IS  - 0950-382X (Print)
IS  - 0950-382X (Linking)
VI  - 35
IP  - 4
DP  - 2000 Feb
TI  - Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 
      transcription factor in budding yeast.
PG  - 825-34
AB  - Pho85 is a cyclin-dependent protein kinase (Cdk) in budding yeast with roles in 
      cell metabolism and cell cycle progression. Activation of Pho85 occurs through 
      association with Pho85 cyclins (Pcls), of which 10 are known. When complexed with 
      the G1 cyclins, Pcl1 and Pcl2, Pho85 is required for cell cycle progression in 
      the absence of the Cdc28-dependent cyclins, Cln1 and Cln2. To identify potential 
      targets of Pcl2-Pho85, we performed a two-hybrid screen using the Pcl2 cyclin as 
      bait and recovered the transcription factor Swi5 as a Pcl2-interacting protein. 
      We performed both biochemical and genetic tests to discover the biological 
      significance of the interaction between Pcl2 and Swi5 seen in the two-hybrid 
      assay. We found that Swi5 interacts in vitro with Pho85 cyclins and is 
      phosphorylated in vitro by the Pho80-Pho85 kinase. We discovered that a subset of 
      genes that are controlled by Swi5 and a homologous transcription factor, Ace2, 
      was misregulated in a pho85 deletion strain; expression of the ASH1 and CTS1 
      genes was reduced in an ace2 deletion strain, whereas expression of both genes 
      was increased in an ace2Delta pho85Delta double mutant. We also found that 
      overexpression of SWI5 caused cell lethality in a pho85 deletion strain. Our 
      results are consistent with misregulation of Swi5 activity in vivo in the absence 
      of Pho85 and implicate Swi5 as a potential substrate of Pho85 cyclin-dependent 
      kinase complexes.
FAU - Measday, V
AU  - Measday V
AD  - Department of Molecular and Medical Genetics, University of Toronto, Rm. 4285 
      Medical Sciences Building, 1 Kings College Circle, Toronto, Ontario, Canada M5S 
      1A8.
FAU - McBride, H
AU  - McBride H
FAU - Moffat, J
AU  - Moffat J
FAU - Stillman, D
AU  - Stillman D
FAU - Andrews, B
AU  - Andrews B
LA  - eng
GR  - 5P30CA42014/CA/NCI NIH HHS/United States
GR  - 5T32GM7464/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (PCL2 protein, S cerevisiae)
RN  - 0 (SWI5 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - EC 2.7.11.22 (PHO85 protein, S cerevisiae)
SB  - IM
MH  - *Cell Cycle Proteins
MH  - Cyclin-Dependent Kinases/genetics/*metabolism
MH  - Cyclins/genetics/metabolism
MH  - *DNA-Binding Proteins
MH  - Fungal Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Fungal
MH  - Mutation
MH  - Phosphorylation
MH  - Protein Binding
MH  - *Saccharomyces cerevisiae Proteins
MH  - Saccharomycetales/genetics/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Two-Hybrid System Techniques
EDAT- 2000/02/26 09:00
MHDA- 2000/04/25 09:00
CRDT- 2000/02/26 09:00
PHST- 2000/02/26 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/02/26 09:00 [entrez]
AID - mmi1754 [pii]
AID - 10.1046/j.1365-2958.2000.01754.x [doi]
PST - ppublish
SO  - Mol Microbiol. 2000 Feb;35(4):825-34. doi: 10.1046/j.1365-2958.2000.01754.x.