PMID- 10696780
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20190513
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 53
IP  - 2
DP  - 2000 Feb
TI  - Serum alters the uptake and relative potencies of halogenated aromatic 
      hydrocarbons in cell culture bioassays.
PG  - 316-25
AB  - The effects of many chemicals on cellular processes are governed by their ability 
      to enter the cell, which is in turn a function of the composition of the cell's 
      external environment. To examine this relationship, the effect of serum in cell 
      culture medium on the bioavailability of cytochrome P450 1A (CYP1A)-inducing 
      compounds was determined in PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) 
      cells. The presence of 10% calf serum in the medium increased the EC50 (effective 
      concentration to achieve 50% maximal response) for induction of ethoxyresorufin 
      O-deethylase (EROD) activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) 
      20-fold as compared to treatment in serum-free medium. Measurement of [3H]TCDD 
      uptake and Ah receptor binding indicated that the apparent difference in 
      potencies was a result of decreased bioavailability in the presence of serum, 
      effectively reducing the concentration of TCDD within the cells. Induction of 
      EROD and CYP1A protein in response to treatment with each of three coplanar 
      polychlorinated biphenyls (PCB congeners 77, 126, and 169) was similarly affected 
      by serum, although the magnitude varied among inducers and assays. Relative 
      potencies (calculated as EC50TCDD / EC50PCB) for EROD induction by the three PCBs 
      were significantly higher in the absence of serum. However, serum showed no 
      significant effect on the relative potencies for CYP1A protein induction. These 
      results demonstrate that measured inducing potencies, and relative potencies for 
      EROD induction, by halogenated aromatic hydrocarbons are strongly dependent on 
      the composition of culture medium, which can lead to artificial differences in 
      comparisons among cell types.
FAU - Hestermann, E V
AU  - Hestermann EV
AD  - Biology Department, Woods Hole Oceanographic Institution, Massachusetts 02543, 
      USA.
FAU - Stegeman, J J
AU  - Stegeman JJ
FAU - Hahn, M E
AU  - Hahn ME
LA  - eng
GR  - R01 ES006272/ES/NIEHS NIH HHS/United States
GR  - R01 ES006272-08/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Biological Availability
MH  - *Blood
MH  - Carcinoma, Hepatocellular/metabolism
MH  - Cattle
MH  - Culture Media, Serum-Free/pharmacology
MH  - Cytochrome P-450 CYP1A1/biosynthesis
MH  - Enzyme Induction/drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Liver Neoplasms/metabolism
MH  - Poecilia
MH  - Polychlorinated Biphenyls/*pharmacokinetics/toxicity
MH  - Polychlorinated Dibenzodioxins/*pharmacokinetics/toxicity
MH  - Receptors, Aryl Hydrocarbon/metabolism
MH  - Tumor Cells, Cultured/*metabolism
EDAT- 2000/03/04 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - 10.1093/toxsci/53.2.316 [doi]
PST - ppublish
SO  - Toxicol Sci. 2000 Feb;53(2):316-25. doi: 10.1093/toxsci/53.2.316.