PMID- 10698207
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20091119
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 141
IP  - 3
DP  - 2000 Mar
TI  - Nuclear receptor coactivators facilitate vitamin D receptor homodimer action on 
      direct repeat hormone response elements.
PG  - 1281-4
AB  - Vitamin D receptor (VDR) is a ligand-dependent transcription factor that 
      regulates target gene expression. Although VDR forms stable heterodimer complex 
      with retinoid X receptors (RXRs) on vitamin D-response elements (VDREs), it is 
      still not clear whether VDR/RXR heterodimers are the only VDR complexes 
      responsible for vitamin D-mediated gene transcription. In this report, we 
      analyzed the effect of nuclear receptor coactivators (SRC-1 and TRAM-1) on VDR 
      homodimer and VDR/RXR heterodimer formation by electrophoretic mobility shift 
      assay. We found that VDR forms stable homodimers after interaction with the 
      coactivators on a VDRE (DR+3). Of particular note, DR+4 and DR+5 hormone-response 
      elements (HREs) may also support such interactions. Cotransfection experiments 
      revealed further that the coactivators enhance ligand-induced VDR transcription 
      on these elements. Our studies suggest the important role of VDR homodimers, in 
      addition to VDR/RXR heterodimers, in vitamin D-induced transactivation. Thus, 
      specific coactivator-VDR interactions on HREs may determine target gene 
      transactivation.
FAU - Takeshita, A
AU  - Takeshita A
AD  - Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 
      Boston, MA 02115, USA. akira18@mail7.dddd.ne.jp
FAU - Ozawa, Y
AU  - Ozawa Y
FAU - Chin, W W
AU  - Chin WW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Ligands)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Thyroid Hormones)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 3)
SB  - IM
MH  - Cell Nucleus/*metabolism
MH  - Electrophoresis
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Ligands
MH  - Nuclear Receptor Coactivator 1
MH  - Nuclear Receptor Coactivator 3
MH  - Plasmids/genetics
MH  - Receptors, Calcitriol/*metabolism
MH  - Receptors, Steroid/genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Response Elements/*genetics
MH  - Thyroid Hormones/physiology
MH  - Transcription Factors/genetics
MH  - Transfection/genetics
EDAT- 2000/03/04 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - 10.1210/endo.141.3.7441 [doi]
PST - ppublish
SO  - Endocrinology. 2000 Mar;141(3):1281-4. doi: 10.1210/endo.141.3.7441.