PMID- 10698943
OWN - NLM
STAT- MEDLINE
DCOM- 20000426
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 5
DP  - 2000 Mar 1
TI  - Hormone activation induces nucleosome positioning in vivo.
PG  - 1023-33
AB  - The mouse mammary tumor virus (MMTV) promoter is induced by glucocorticoid 
      hormone. A robust hormone- and receptor-dependent activation could be reproduced 
      in Xenopus laevis oocytes. The homogeneous response in this system allowed a 
      detailed analysis of the transition in chromatin structure following hormone 
      activation. This revealed two novel findings: hormone activation led to the 
      establishment of specific translational positioning of nucleosomes despite the 
      lack of significant positioning in the inactive state; and, in the active 
      promoter, a subnucleosomal particle encompassing the glucocorticoid receptor 
      (GR)-binding region was detected. The presence of only a single GR-binding site 
      was sufficient for the structural transition to occur. Both basal promoter 
      elements and ongoing transcription were dispensable. These data reveal a stepwise 
      process in the transcriptional activation by glucocorticoid hormone.
FAU - Belikov, S
AU  - Belikov S
AD  - Laboratory of Molecular Genetics, Department of Cell and Molecular Biology, 
      Medical Nobel Institute, Karolinska Institutet, SE-17177 Stockholm, Sweden.
FAU - Gelius, B
AU  - Gelius B
FAU - Almouzni, G
AU  - Almouzni G
FAU - Wrange, O
AU  - Wrange O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Glucocorticoids)
RN  - 0 (Nucleosomes)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression Regulation
MH  - Glucocorticoids/*genetics/metabolism
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Nucleosomes/*genetics/metabolism
MH  - Oocytes
MH  - Promoter Regions, Genetic
MH  - Xenopus laevis
PMC - PMC305641
EDAT- 2000/03/04 09:00
MHDA- 2000/04/29 09:00
PMCR- 2001/03/01
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
PHST- 2001/03/01 00:00 [pmc-release]
AID - 191023 [pii]
AID - 10.1093/emboj/19.5.1023 [doi]
PST - ppublish
SO  - EMBO J. 2000 Mar 1;19(5):1023-33. doi: 10.1093/emboj/19.5.1023.