PMID- 10699668
OWN - NLM
STAT- MEDLINE
DCOM- 20000414
LR  - 20191103
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 40
IP  - 2
DP  - 2000 Apr
TI  - Widefield deconvolution epifluorescence microscopy combined with fluorescence in 
      situ hybridization reveals the spatial arrangement of bacteria in sponge tissue.
PG  - 125-34
AB  - Widefield deconvolution epifluorescence microscopy (WDEM) combined with 
      fluorescence in situ hybridization (FISH) was performed to identify and 
      characterize single bacterial cells within sections of the mediterranean sponge 
      Chondrosia reniformis. Sponges were embedded in paraffin wax or plastic prior to 
      the preparation of thin sections, in situ hybridization and microscopy. Serial 
      digital images generated by widefield epifluorescence microscopy were visualized 
      using an exhaustive photon reassignment deconvolution algorithm and 
      three-dimensional rendering software. Computer processing of series of images 
      taken at different focal planes with the deconvolution technique provided 
      deblurred three-dimensional images with high optical resolution on a submicron 
      scale. Results from the deconvolution enhanced widefield microscopy were compared 
      with conventional epifluorescent microscopical images. By the application of the 
      deconvolution algorithm on digital image data obtained with widefield 
      epifluorescence microscopy after FISH, the occurrence and spatial arrangement of 
      Desulfovibrionaceae closely associated with micropores of Chondrosia reniformis 
      could be visualized.
FAU - Manz, W
AU  - Manz W
AD  - Technische Universitat Berlin, Institut fur Technischen Umweltschutz, Fachgebiet 
      Okologie der Mikroorganismen, Sekretariat OE 5, Franklinstrasse 29, D-10587, 
      Berlin, Germany. manz0654@mailszrz.tu-berlin.de
FAU - Arp, G
AU  - Arp G
FAU - Schumann-Kindel, G
AU  - Schumann-Kindel G
FAU - Szewzyk, U
AU  - Szewzyk U
FAU - Reitner, J
AU  - Reitner J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Algorithms
MH  - Animals
MH  - Image Enhancement/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Microtomy
MH  - Oligonucleotide Probes
MH  - Porifera/*microbiology/ultrastructure
MH  - Proteobacteria/*ultrastructure
MH  - Tissue Embedding
EDAT- 2000/03/04 09:00
MHDA- 2000/04/25 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - S0167-7012(99)00103-7 [pii]
AID - 10.1016/s0167-7012(99)00103-7 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2000 Apr;40(2):125-34. doi: 10.1016/s0167-7012(99)00103-7.