PMID- 10700561
OWN - NLM
STAT- MEDLINE
DCOM- 20000504
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 857
IP  - 1-2
DP  - 2000 Feb 28
TI  - Brain-derived neurotrophic factor requirement for activity-dependent maturation 
      of glutamatergic synapse in developing mouse somatosensory cortex.
PG  - 141-50
AB  - The maturation of cortical circuitry critically depends on experience. Recently, 
      a model of silent synapse has been proposed as a mechanism of activity-mediated 
      transition of immature synapse to mature synapse. It is not clear, however, how 
      activity could regulate this transition. Here, we show the evidence that 
      endogenous brain-derived neurotrophic factor (BDNF) is required for the 
      maturation of glutamatergic synapse in developing mouse somatosensory cortex. 
      Field potential recordings of thalamocortical glutamatergic synaptic activity 
      with brain slices from the BDNF mutant mice showed that AMPA receptor responses 
      are low, but NMDA receptor responses remain high in layer 4, thus, the relative 
      contribution of AMPA receptor response is significantly lower compared to the 
      age-matched wild-type mouse. Furthermore, optical images of development of 
      thalamocortical connectivity with a voltage-sensitive dye showed that NMDA 
      receptor-dominant synapse is established first in layer 4 and layer 5/6 then AMPA 
      receptor response appears later in concomitant with reduction of NMDA receptor 
      response in layer 4 and that the maturation of the silent synapse is impaired in 
      the BDNF mutant mice. In layer 5/6, NMDA receptor response was suppressed without 
      upregulation of AMPA receptor response. This process also required BDNF function. 
      Interestingly, whisker-trimming of the wild-type mouse from just after birth 
      showed quite similar results with the homozygous mutant of their whiskers left 
      intact. Therefore, we would propose that BDNF is a critical mediator for the 
      maturation of glutamatergic synapse in developing mouse somatosensory cortex.
FAU - Itami, C
AU  - Itami C
AD  - Division of Biochemistry and Cellular Biology, National Institute of 
      Neuroscience, 4-1-1, Ogawahigashi, Kodaira, Japan.
FAU - Mizuno, K
AU  - Mizuno K
FAU - Kohno, T
AU  - Kohno T
FAU - Nakamura, S
AU  - Nakamura S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Receptors, AMPA)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Glutamic Acid/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Mutation
MH  - Nerve Growth Factors/genetics/metabolism
MH  - Neural Pathways/cytology/growth & development/metabolism
MH  - Neurons/chemistry/cytology/metabolism
MH  - Receptors, AMPA/drug effects/metabolism
MH  - Somatosensory Cortex/cytology/*growth & development/*metabolism
MH  - Synapses/*metabolism/ultrastructure
MH  - Thalamus/cytology/growth & development/metabolism
EDAT- 2000/03/04 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - S0006-8993(99)02352-5 [pii]
AID - 10.1016/s0006-8993(99)02352-5 [doi]
PST - ppublish
SO  - Brain Res. 2000 Feb 28;857(1-2):141-50. doi: 10.1016/s0006-8993(99)02352-5.