PMID- 10700858
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 117
IP  - 1
DP  - 2000 Feb
TI  - Autologous transplantation of in vivo purged PBSC in CML: comparison of FISH, 
      cytogenetics, and PCR detection of Philadelphia chromosome in leukapheresis 
      products.
PG  - 1-8
AB  - To determine the effectiveness of different methods for the detection of tumor 
      cell contamination of collected peripheral stem cells, we performed a study on 39 
      chronic myelogenous leukemia (CML) patients who were consecutively treated at our 
      department. Analyses of tumor cell contamination by fluorescence in situ 
      hybridization (FISH), conventional cytogenetics, and polymerase chain reaction 
      (PCR) showed marked differences in the percentage of evaluable results: 
      Quantitative analysis of tumor cell contamination was feasible in 60 of 105 (57%) 
      samples evaluated with the use of conventional cytogenetic analysis and in 105 of 
      107 (98%) samples analyzed by FISH. PCR was evaluable in all 85 samples tested 
      (100%). Both methods were shown to be adequate overall in determining the number 
      of BCR-ABL positive cells, although cytogenetics tended to produce slightly 
      higher percentages. Based on these results, we conclude that FISH performed on 
      leukapheresis products is a rapid and reliable method for assessing the quality 
      of these products and should be used for routine evaluation of tumor cell 
      contamination of CML stem cell products.
FAU - Hess, G
AU  - Hess G
AD  - III Medizinische Klinik, Johannes Gutenberg-Universitat, Mainz, Germany.
FAU - Reifenrath, C
AU  - Reifenrath C
FAU - Friedrich-Freksa, A
AU  - Friedrich-Freksa A
FAU - Beyer, V
AU  - Beyer V
FAU - Naumann, S
AU  - Naumann S
FAU - Schuch, B
AU  - Schuch B
FAU - Huber, C
AU  - Huber C
FAU - Fischer, T
AU  - Fischer T
FAU - Decker, H J
AU  - Decker HJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Primers)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - *Bone Marrow Purging
MH  - DNA Primers
MH  - Female
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukapheresis
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*pathology/therapy
MH  - Male
MH  - Middle Aged
MH  - *Philadelphia Chromosome
MH  - Polymerase Chain Reaction
MH  - Transplantation, Autologous
EDAT- 2000/03/04 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - S0165-4608(99)00133-8 [pii]
AID - 10.1016/s0165-4608(99)00133-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Feb;117(1):1-8. doi: 10.1016/s0165-4608(99)00133-8.