PMID- 10700866
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 117
IP  - 1
DP  - 2000 Feb
TI  - Fluorescence in situ hybridization confirmation of 5q deletions in patients with 
      hematological malignancies.
PG  - 45-9
AB  - Fluorescence in situ hybridization (FISH) using specific probes for the 5q31-32 
      region and a whole chromosomal painting (WCP) probe for chromosome 5 were used to 
      corroborate the results of classical cytogenetic examinations performed on 
      G-banded chromosomes of 77 patients with hematological malignancies. Using 
      classical cytogenetic methods, we suspected the presence of clones with a 
      deletion 5q in 63 patients, and complex rearrangements with involvement of 
      chromosome 5 in 14 other cases. Fluorescence in situ hybridization proved the 
      occurrence of deletion 5q31 in 23 patients and ascertained translocations of part 
      of the long arms of deleted chromosome 5 with missing region 5q31 in 12 patients. 
      In 2 cases, the 5q31 region was translocated to other chromosomes as a part of 
      complex rearrangements. The combination of classical cytogenetics and FISH with 
      specific probes for the 5q31 band yielded cytogenetic results in 35 cases. 
      Routine FISH detection of deleted regions was possible by commercially available 
      cosmid probes for the 5q31 chromosomal band. The interpretation of small 
      deletions and frequent involvement of the deleted chromosomes 5 in complex 
      translocations were ascertained by WCP probes.
FAU - Brezinova, J
AU  - Brezinova J
AD  - Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
FAU - Zemanova, Z
AU  - Zemanova Z
FAU - Cermak, J
AU  - Cermak J
FAU - Michalova, K
AU  - Michalova K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 5
MH  - Female
MH  - Hematologic Neoplasms/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mitosis
EDAT- 2000/03/04 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - S0165-4608(99)00142-9 [pii]
AID - 10.1016/s0165-4608(99)00142-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Feb;117(1):45-9. doi: 10.1016/s0165-4608(99)00142-9.