PMID- 10700870
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 117
IP  - 1
DP  - 2000 Feb
TI  - A broad amplification pattern at 3q in squamous cell lung cancer--a fluorescence 
      in situ hybridization study.
PG  - 66-70
AB  - Frequent DNA copy number gain at 3q, with minimal overlapping area at 3q24-qter, 
      has previously been reported in squamous cell carcinoma of the lung (SQCC), 
      implicating the importance of genes at 3q in the tumorigenesis of SQCC. To 
      further characterize the gain of DNA sequences at 3q, we performed interphase 
      fluorescence in situ hybridization (FISH) analysis on 16 paraffin-embedded SQCC 
      tumor samples that had previously been studied by comparative genomic 
      hybridization (CGH). Eleven yeast artificial chromosome (YAC) probes located at 
      3q25-q27 and a chromosome 3-specific centromeric probe were used in the analysis. 
      All SQCC tumors showed increase in DNA sequence copy number with 9-11 probes. In 
      5 tumors (31%) the number of centromeric signals varied from 3 to 5 and the 
      YAC/centromeric signal ratio was 1.0, suggesting that the increase in DNA 
      sequence copy number at 3q in these cases resulted from polysomy of chromosome 3. 
      In 11 tumors (69%), the YAC/centromeric signal ratio varied between 1.5 and 4.7, 
      indicating that the increase in DNA sequence copy number was due to 
      intrachromosomal gain of DNA sequences at 3q. In each case, several YACs showed 
      increased number of signals, demonstrating that the gained area was relatively 
      large. Our findings therefore suggest that multiple genes located at 3q25-q27 are 
      involved in the tumorigenesis of SQCC.
FAU - Kettunen, E
AU  - Kettunen E
AD  - Department of Medical Genetics, Haartman Institute, Helsinki, Finland.
FAU - el-Rifai, W
AU  - el-Rifai W
FAU - Bjorkqvist, A M
AU  - Bjorkqvist AM
FAU - Wolff, H
AU  - Wolff H
FAU - Karjalainen, A
AU  - Karjalainen A
FAU - Anttila, S
AU  - Anttila S
FAU - Mattson, K
AU  - Mattson K
FAU - Husgafvel-Pursiainen, K
AU  - Husgafvel-Pursiainen K
FAU - Knuutila, S
AU  - Knuutila S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Centromere
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 3
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics
EDAT- 2000/03/04 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - S0165-4608(99)00146-6 [pii]
AID - 10.1016/s0165-4608(99)00146-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Feb;117(1):66-70. doi: 
      10.1016/s0165-4608(99)00146-6.