PMID- 10701129 OWN - NLM STAT- MEDLINE DCOM- 20000420 LR - 20190915 IS - 0831-2796 (Print) IS - 0831-2796 (Linking) VI - 43 IP - 1 DP - 2000 Feb TI - Isolation, characterization, and chromosomal location of the tRNA(Met) genes in Atlantic salmon (Salmo salar) and brown trout (Salmo trutta). PG - 185-90 AB - This work describes the isolation, characterization, and physical location of the methionine tRNA in the genome of Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.). An Atlantic salmon genomic library was screened using a tRNA(Met) probe from Xenopus laevis. Two cosmid clones containing the Atlantic salmon tRNA(Met) gene were isolated, subcloned and sequenced. The tRNA(Met) was mapped to metaphase chromosomes by fluorescence in situ hybridization (FISH). Chromosomal data indicated that the tDNA of methionine is tandemly repeated in a single locus in both species. Analysis of genomic DNA by Southern hybridization confirmed the tandem organization of this gene. FAU - Perez, J AU - Perez J AD - Departamento de Biologia Funcional, Facultad de Medicina, Universidad de Oviedo, Spain. FAU - Moran, P AU - Moran P FAU - Garcia-Vazquez, E AU - Garcia-Vazquez E LA - eng SI - GENBANK/AJ131198 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Canada TA - Genome JT - Genome JID - 8704544 RN - 0 (RNA, Transfer, Met) SB - IM MH - Animals MH - Base Sequence MH - Chromosome Mapping MH - *Genes MH - In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - RNA, Transfer, Met/*genetics MH - Salmo salar/genetics MH - Salmon/*genetics MH - Species Specificity EDAT- 2000/03/04 09:00 MHDA- 2000/04/25 09:00 CRDT- 2000/03/04 09:00 PHST- 2000/03/04 09:00 [pubmed] PHST- 2000/04/25 09:00 [medline] PHST- 2000/03/04 09:00 [entrez] AID - 10.1139/g99-084 [doi] PST - ppublish SO - Genome. 2000 Feb;43(1):185-90. doi: 10.1139/g99-084.