PMID- 10701130 OWN - NLM STAT- MEDLINE DCOM- 20000420 LR - 20161020 IS - 0831-2796 (Print) IS - 0831-2796 (Linking) VI - 43 IP - 1 DP - 2000 Feb TI - Physical characterization of the homoeologous group 5 chromosomes of wheat in terms of rice linkage blocks, and physical mapping of some important genes. PG - 191-8 AB - The wheat homoeologous Group 5 chromosomes were characterized physically in terms of rice linkage blocks using a deletion mapping approach. All three chromosomes, 5A, 5B, and 5D, were shown to have a similar structure, apart from the 4A-5A translocation on the distal end of chromosome arm 5AL. The physical mapping of rice markers on the deletion lines revealed that the whole of rice chromosome 9 is syntenous to a large block, proximal to the centromere, on the long arm. Likewise, a small segment of the distal end of the long arm showed conserved synteny with the distal one-third end of the long arm of rice chromosome 3. In between those conserved regions, there is a region on the long arm of the Group 5 chromosomes which shows broken synteny. The proximal part of the short arms of the Group 5 chromosomes showed conserved synteny with a segment of the short arm of rice chromosome 11 and the distal ends showed conserved synteny with a segment of rice chromosome 12. The physical locations of flowering time genes (Vrn and earliness per se) and the gene for grain hardness (Ha) on the Group 5 chromosomes were determined. These results indicate that comparative mapping using the deletion mapping approach is useful in the study of genome relationships, the physical location of genes, and can determine the appropriate gene cloning strategy. FAU - Sarma, R N AU - Sarma RN AD - John Innes Centre, Norwich Research Park, Norwich, U.K. FAU - Fish, L AU - Fish L FAU - Gill, B S AU - Gill BS FAU - Snape, J W AU - Snape JW LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Canada TA - Genome JT - Genome JID - 8704544 SB - IM MH - Chromosome Mapping MH - Cloning, Molecular MH - Genes, Plant MH - Oryza/genetics MH - Periodicity MH - Sequence Deletion MH - Species Specificity MH - Triticum/*genetics EDAT- 2000/03/04 09:00 MHDA- 2000/04/25 09:00 CRDT- 2000/03/04 09:00 PHST- 2000/03/04 09:00 [pubmed] PHST- 2000/04/25 09:00 [medline] PHST- 2000/03/04 09:00 [entrez] PST - ppublish SO - Genome. 2000 Feb;43(1):191-8.