PMID- 10701131
OWN - NLM
STAT- MEDLINE
DCOM- 20000420
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 43
IP  - 1
DP  - 2000 Feb
TI  - Construction of a bacterial artificial chromosome (BAC) library for potato 
      molecular cytogenetics research.
PG  - 199-204
AB  - Lack of reliable techniques for chromosome identification is the major obstacle 
      for cytogenetics research in plant species with large numbers of small 
      chromosomes. To promote molecular cytogenetics research of potato (Solanum 
      tuberosum, 2n = 4x = 48) we developed a bacterial artificial chromosome (BAC) 
      library of a diploid potato species S. bulbocastanum. The library consists of 
      23,808 clones with an average insert size of 155 kb, and represents approximately 
      3.7 equivalents to the potato genome. The majority of the clones in the BAC 
      library generated distinct signals on specific potato chromosomes using 
      fluorescence in situ hybridization (FISH). The hybridization signals provide 
      excellent cytological markers to tag individual potato chromosomes. We also 
      demonstrated that the BAC clones can be mapped to specific positions on meiotic 
      pachytene chromosomes. The excellent resolution of pachytene FISH can be used to 
      construct a physical map of potato by mapping molecular marker-targeted BAC 
      clones on pachytene chromosomes.
FAU - Song, J
AU  - Song J
AD  - Department of Horticulture, University of Wisconsin-Madison 53706, USA.
FAU - Dong, F
AU  - Dong F
FAU - Jiang, J
AU  - Jiang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Bacterial/*genetics
MH  - Cloning, Molecular
MH  - Cytogenetic Analysis
MH  - DNA, Plant/genetics
MH  - *Gene Library
MH  - Genetic Markers
MH  - Genetic Vectors/*genetics
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis
MH  - Solanum tuberosum/*genetics
EDAT- 2000/03/04 09:00
MHDA- 2000/04/25 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
PST - ppublish
SO  - Genome. 2000 Feb;43(1):199-204.