PMID- 10701824
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190905
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 418
IP  - 3
DP  - 2000 Mar 13
TI  - Expression of nerve growth factor, brain-derived neurotrophic factor, and 
      neurotrophin-3 in the somatosensory cortex of the mature rat: coexpression with 
      high-affinity neurotrophin receptors.
PG  - 241-54
AB  - Neurotrophins, including nerve growth factor (NGF), brain-derived neurotrophic 
      factor (BDNF), and neurotrophin-3 (NT-3), are critical for the maintenance and 
      plasticity of central nervous system (CNS) neurons. We tested the hypothesis that 
      cortical neurons participate in redundant autocrine/paracrine systems. Three sets 
      of studies determined the distribution of NGF-, BDNF-, and NT-3-expressing 
      neurons, the frequency of neurons coexpressing NGF and BDNF, and the frequency of 
      neurons expressing a neurotrophin and its associated high-affinity receptor. The 
      distribution of NGF-, BDNF, and NT-3-immunoreactive neurons was identical. 
      Neurotrophin-positive cells were parceled throughout the cortex, although the 
      labeling frequency was not the same in all layers. More than 30% of the neurons 
      in layers II/III, V, and VI were labeled, whereas only 5-10% of the neurons in 
      layer IV was immunopositive for a neurotrophin. Some glia were also neurotrophin 
      positive, particularly BDNF-positive glia. About 70% of the neurons in layers 
      II/III and V coexpressed NGF and BDNF or coexpressed NGF and NT-3. 
      Ligand-receptor colabeling was also common among cortical neurons. For example, 
      nearly 70% of the NGF-, BDNF-, and NT-3-positive neurons in layer V colabeled 
      with their respective high-affinity receptors, i.e., trkA, trkB, and trkC, 
      respectively. Thus, (a) neurons express multiple neurotrophins and (b) cortical 
      neurons (e.g., layer V neurons) contain the components required for 
      autocrine/paracrine and/or anterograde communication (e.g., neurons in layer 
      II/III support layer V neurons). These systems mean that the cortex is capable of 
      regulating itself autonomously.
FAU - Pitts, A F
AU  - Pitts AF
AD  - Veterans Affairs Medical Center, and Department of Psychiatry, University of Iowa 
      College of Medicine, Iowa City 52246-2208, USA.
FAU - Miller, M W
AU  - Miller MW
LA  - eng
GR  - AA06916/AA/NIAAA NIH HHS/United States
GR  - AA07568/AA/NIAAA NIH HHS/United States
GR  - AA09616/AA/NIAAA NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Receptors, Nerve Growth Factor)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Binding, Competitive
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Nerve Growth Factors/*metabolism
MH  - Neurotrophin 3/*metabolism
MH  - Rats/*metabolism
MH  - Rats, Long-Evans
MH  - Receptors, Nerve Growth Factor/metabolism
MH  - Somatosensory Cortex/*metabolism
EDAT- 2000/03/04 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/03/04 09:00
PHST- 2000/03/04 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/03/04 09:00 [entrez]
AID - 10.1002/(SICI)1096-9861(20000313)418:3<241::AID-CNE1>3.0.CO;2-M [pii]
AID - 10.1002/(sici)1096-9861(20000313)418:3<241::aid-cne1>3.0.co;2-m [doi]
PST - ppublish
SO  - J Comp Neurol. 2000 Mar 13;418(3):241-54. doi: 
      10.1002/(sici)1096-9861(20000313)418:3<241::aid-cne1>3.0.co;2-m.