PMID- 10702366
OWN - NLM
STAT- MEDLINE
DCOM- 20000410
LR  - 20131121
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 163
IP  - 3
DP  - 2000 Mar 15
TI  - Chloride secretion in kidney distal epithelial cells (A6) evoked by cadmium.
PG  - 267-78
AB  - The effect of Cd(2+) on chloride secretion was examined in A6 renal epithelia 
      cells by chloride-sensitive fluorescence (SPQ probe) and by the 
      short-circuit-current (I(sc)) technique. Depleting the cells of Cl(-) suggests 
      that the Cd(2+)-activated I(sc) (DeltaI(sc(Cd))) is dependent on the presence of 
      Cl(-) ions. Among the Cl(-)-channel inhibitors the fenemates, flufenamic acid 
      (FFA) and niflumic acid (NFA), and 5-nitro-2-(3-phenylpropylamino)-benzoate 
      (NPPB) significantly lowered DeltaI(sc(Cd)) compared with control level. In 
      SPQ-loaded A6 cells, Cd(2+) evoked an increase in Cl(-) secretion 
      ([DeltaCl(-)](Cd)), which significantly exceeded the basal Cl(-) transport and 
      was blockable by FFA and NFA. The closely related metals, Zn(2+) or Ni(2+), were 
      also able to activate Cl(-) secretion. Preexposure of Zn(2+) or Ni(2+) completely 
      prevented [DeltaCl(-)](Cd), suggesting that Zn(2+) and Ni(2+) probably use 
      similar mechanisms. Like Cd(2+), thapsigargin (TG), an inhibitor of intracellular 
      Ca(2+)-ATPase and the Ca(2+)-ionophore A23187, induced an increase in I(sc). 
      Moreover, TG and Cd(2+) were able to neutralize the responses of the counterparts 
      as also observed in I(sc) measurements, which indicates that Cd(2+) activates 
      Cl(-) secretion in a Ca(2+)-dependent manner. Hence, this study supports the idea 
      that basolateral Cd(2+) (possibly also Zn(2+) and Ni(2+)), probably through a 
      Ca(2+)-sensing receptor, causes calcium mobilization that activates apical 
      fenemate-sensitive chloride channels leading to chloride secretion in A6 cells.
CI  - Copyright 2000 Academic Press.
FAU - Faurskov, B
AU  - Faurskov B
AD  - Grenaa Central Hospital, Sygehusuej 6, 8500, Grenaa, Denmark. bf@get2net.dk
FAU - Bjerregaard, H F
AU  - Bjerregaard HF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Chloride Channels)
RN  - 0 (Chlorides)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Metals)
RN  - 00BH33GNGH (Cadmium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Algorithms
MH  - Animals
MH  - Cadmium/*toxicity
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Chloride Channels/antagonists & inhibitors/metabolism
MH  - Chlorides/*metabolism
MH  - Electrophysiology
MH  - Epithelial Cells/drug effects/metabolism
MH  - Fluorescent Dyes
MH  - Kidney/cytology/drug effects/*metabolism
MH  - Metals/toxicity
MH  - Patch-Clamp Techniques
MH  - Spectrometry, Fluorescence
MH  - Xenopus laevis
EDAT- 2000/03/07 09:00
MHDA- 2000/04/15 09:00
CRDT- 2000/03/07 09:00
PHST- 2000/03/07 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/03/07 09:00 [entrez]
AID - S0041-008X(99)98852-X [pii]
AID - 10.1006/taap.1999.8852 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2000 Mar 15;163(3):267-78. doi: 10.1006/taap.1999.8852.