PMID- 10702489
OWN - NLM
STAT- MEDLINE
DCOM- 20000428
LR  - 20210526
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 7
IP  - 2
DP  - 2000 Mar
TI  - Anti-idiotypic antibodies in patients with different clinical forms of 
      paracoccidioidomycosis.
PG  - 175-81
AB  - Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin 
      America. Patients with PCM show a wide spectrum of clinical and pathological 
      manifestations depending on both host and pathogen factors. Two clinical forms of 
      the disease are recognized: the acute or juvenile form and the chronic or adult 
      form. The major antigenic component of the parasite is a glycoprotein of 43 kDa 
      (gp43). All patient sera present antibodies against gp43 (anti-gp43) and, as 
      demonstrated before by our group, spontaneous anti-idiotypic (anti-Id) antibodies 
      (Ab2) can be detected in patient sera with high titers of anti-gp43. Since it has 
      been postulated that anti-Id antibodies may have a modulating function, we 
      decided to purify and characterize anti-Id antibodies in this system. The 
      possible correlation of Ab2 titers with different clinical forms of disease was 
      also verified. Results showed that purified human anti-Id antibodies (human Ab2) 
      recognized specifically the idiotype of some murine monoclonal anti-gp43 (17c and 
      3e) but not others (40.d7, 27a, and 8a). Spontaneous anti-Id antibodies were 
      found in all clinical forms of disease. The majority of patients (88%, n = 8) 
      with the acute form of PCM had high titers of Ab2. However, among patients with 
      the multifocal chronic form of the disease, only 29% (n = 14) had high titers of 
      Ab2; 70% (n = 10) of patients with the unifocal chronic form had low titers of 
      Ab2. A correlation between Ab2 titers and anti-gp43 titers was observed before 
      and during antimycotic treatment. Our results suggest that titers of anti-Id 
      antibodies correlate with the severity of PCM in humans.
FAU - Souza, A R
AU  - Souza AR
AD  - Discipline of Immunology, Federal University of Sao Paulo (UNIFESP), Sao Paulo 
      Brazil.
FAU - Gesztesi, J L
AU  - Gesztesi JL
FAU - del Negro, G M
AU  - del Negro GM
FAU - Benard, G
AU  - Benard G
FAU - Sato, J
AU  - Sato J
FAU - Santos, M V
AU  - Santos MV
FAU - Abrahao, T B
AU  - Abrahao TB
FAU - Lopes, J D
AU  - Lopes JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antifungal Agents)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Oligosaccharides)
RN  - 304NUG5GF4 (Itraconazole)
RN  - 8VZV102JFY (Fluconazole)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/blood/*immunology/isolation & purification
MH  - Antibodies, Fungal/blood/*immunology/isolation & purification
MH  - Antibodies, Monoclonal/immunology/isolation & purification
MH  - Antifungal Agents/therapeutic use
MH  - *Antigens, Fungal
MH  - Fluconazole/therapeutic use
MH  - Follow-Up Studies
MH  - *Fungal Proteins
MH  - Glycoproteins/*immunology
MH  - Humans
MH  - Itraconazole/therapeutic use
MH  - Mice
MH  - Oligosaccharides/*immunology
MH  - Paracoccidioides/immunology
MH  - Paracoccidioidomycosis/blood/drug therapy/*immunology/physiopathology
PMC - PMC95845
EDAT- 2000/03/07 09:00
MHDA- 2000/05/08 09:00
PMCR- 2000/03/01
CRDT- 2000/03/07 09:00
PHST- 2000/03/07 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/03/07 09:00 [entrez]
PHST- 2000/03/01 00:00 [pmc-release]
AID - 0122 [pii]
AID - 10.1128/CDLI.7.2.175-181.2000 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2000 Mar;7(2):175-81. doi: 10.1128/CDLI.7.2.175-181.2000.