PMID- 10704366
OWN - NLM
STAT- MEDLINE
DCOM- 20000623
LR  - 20231213
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 113 ( Pt 7)
DP  - 2000 Apr
TI  - Chromosome condensation induced by geminivirus infection of mature plant cells.
PG  - 1149-60
AB  - Tomato golden mosaic virus (TGMV) is a geminivirus that replicates its 
      single-stranded DNA genome through double-stranded DNA intermediates in nuclei of 
      differentiated plant cells using host replication machinery. We analyzed the 
      distribution of viral and plant DNA in nuclei of infected leaves using 
      fluorescence in situ hybridization (FISH). TGMV-infected nuclei showed up to a 
      sixfold increase in total volume and displayed a variety of viral DNA 
      accumulation patterns. The most striking viral DNA patterns were bright, discrete 
      intranuclear compartments, but diffuse nuclear localization was also observed. 
      Quantitative and spatial measurements of high resolution 3-dimensional image data 
      revealed that these compartments accounted for 1-18% of the total nuclear volume 
      or 2-45% of the total nuclear FISH signals. In contrast, plant DNA was 
      concentrated around the nuclear periphery. In a significant number of nuclei, the 
      peripheral chromatin was organized as condensed prophase-like fibers. A 
      combination of FISH analysis and indirect immunofluorescence with viral coat 
      protein antibodies revealed that TGMV virions are associated with the viral DNA 
      compartments. However, the coat protein antibodies failed to cross react with 
      some large viral DNA inclusions, suggesting that encapsidation may occur after 
      significant viral DNA accumulation. Infection by a TGMV mutant with a defective 
      coat protein open reading frame resulted in fewer and smaller viral 
      DNA-containing compartments. Nevertheless, nuclei infected with the mutant virus 
      increased in size and in some cases showed chromosome condensation. Together, 
      these results established that geminivirus infection alters nuclear architecture 
      and can induce plant chromatin condensation characteristic of cells arrested in 
      early mitosis.
FAU - Bass, H W
AU  - Bass HW
AD  - Department of Biological Science, Florida State University, Tallahassee, FL 
      32306-4370, USA. bass@bio.fsu.edu
FAU - Nagar, S
AU  - Nagar S
FAU - Hanley-Bowdoin, L
AU  - Hanley-Bowdoin L
FAU - Robertson, D
AU  - Robertson D
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Chromatin)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Capsid/biosynthesis/genetics
MH  - Cell Differentiation
MH  - Cell Nucleus/metabolism/physiology/virology
MH  - Chromatin/metabolism
MH  - Chromosomes/*metabolism
MH  - DNA, Single-Stranded/metabolism
MH  - DNA, Viral/metabolism
MH  - Geminiviridae/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Leaves/cytology/genetics/metabolism/virology
MH  - *Plants, Toxic
MH  - Nicotiana/*cytology/genetics/metabolism/*virology
OTO - NASA
OT  - Non-programmatic
EDAT- 2000/03/08 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/03/08 09:00
PHST- 2000/03/08 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/03/08 09:00 [entrez]
AID - 10.1242/jcs.113.7.1149 [doi]
PST - ppublish
SO  - J Cell Sci. 2000 Apr;113 ( Pt 7):1149-60. doi: 10.1242/jcs.113.7.1149.