PMID- 10704688 OWN - NLM STAT- MEDLINE DCOM- 20000317 LR - 20190816 IS - 0165-4608 (Print) IS - 0165-4608 (Linking) VI - 117 IP - 2 DP - 2000 Mar TI - Characterization of a chromosomally complex lung cancer cell line using multiwell fluorescence in situ hybridization. PG - 149-52 AB - The chromosomal characterization of a non-small cell lung cancer cell line (NCIH358) is described. This characterization was achieved using a simple, cheap and technically straightforward multiwell fluorescence in situ hybridization (FISH) method. The many and complex chromosome rearrangements identified by this method could not be defined using conventional G-banded chromosome analysis, and have not been previously described. For the detailed characterization of complex cell lines, multiwell FISH has many advantages over more technically demanding and expensive FISH techniques, and opens up the possibility of screening for consistent rearrangements, leading to the identification of unique fusion genes. FAU - Ogilvie, C M AU - Ogilvie CM AD - Department of Cytogenetics, Guy's & St. Thomas' Hospital Trust, London, United Kingdom. FAU - Shemilt, S AU - Shemilt S FAU - Davies, A F AU - Davies AF FAU - Weber-Hall, S AU - Weber-Hall S FAU - Chuang, C AU - Chuang C FAU - Sundaresan, V AU - Sundaresan V LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cancer Genet Cytogenet JT - Cancer genetics and cytogenetics JID - 7909240 SB - IM MH - Carcinoma, Non-Small-Cell Lung/*genetics/pathology MH - *Chromosome Aberrations MH - Humans MH - In Situ Hybridization/*methods MH - Karyotyping MH - Lung Neoplasms/*genetics/pathology MH - Tumor Cells, Cultured EDAT- 2000/03/08 09:00 MHDA- 2000/03/25 09:00 CRDT- 2000/03/08 09:00 PHST- 2000/03/08 09:00 [pubmed] PHST- 2000/03/25 09:00 [medline] PHST- 2000/03/08 09:00 [entrez] AID - S016546089900148X [pii] AID - 10.1016/s0165-4608(99)00148-x [doi] PST - ppublish SO - Cancer Genet Cytogenet. 2000 Mar;117(2):149-52. doi: 10.1016/s0165-4608(99)00148-x.