PMID- 10705298
OWN - NLM
STAT- MEDLINE
DCOM- 20000405
LR  - 20061115
IS  - 1148-5493 (Print)
IS  - 1148-5493 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Mar
TI  - Interleukin-18 stimulates HIV-1 replication in a T-cell line.
PG  - 47-52
AB  - Interleukin-18 (IL-18) is a recently identified proinflammatory cytokine. Its 
      ability to induce interferon-g suggests a potential virustatic effect. On the 
      other hand, it stimulates NFkB - an activator of HIV replication. Recently, 
      stimulation of HIV-1 in monocytic cells has been demonstrated. In the present 
      study, the influence of IL-18 on HIV-1 replication in lymphatic cells was 
      investigated. Hut78 cells were infected with HIV-1 in the presence of recombinant 
      human IL-18 expressed either in E. coli or eucaryotically by baculovirus in Sf9 
      cells. HIV-1 replication was monitored by p24 ELISA and endpoint titration of 
      culture supernatants on C8166 cells. The addition of IL-18 led to a 3- to 15-fold 
      enhancement of HIV replication in Hut78 cells. By addition of neutralising 
      monoclonal anti-IL-18 antibodies, this effect of IL-18 was reduced by 75%. 
      Exposure of Hut78 to IL-18 prior to HIV infection could exclude the possibility 
      that IL-18 promotes infection of cells. Taken together, these data provide direct 
      evidence for an IL-18-mediated enhancement of HIV-1 replication in lymphatic 
      cells.
FAU - Klein, S A
AU  - Klein SA
AD  - Universitatsklinik Frankfurt, Medizinische Klinik III, Theodor-Stern-Kai 7, 
      D-60590 Frankfurt, Germany.
FAU - Klebba, C
AU  - Klebba C
FAU - Kauschat, D
AU  - Kauschat D
FAU - Pape, M
AU  - Pape M
FAU - Ozmen, L
AU  - Ozmen L
FAU - Hoelzer, D
AU  - Hoelzer D
FAU - Ottmann, O G
AU  - Ottmann OG
FAU - Kalina, U
AU  - Kalina U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur Cytokine Netw
JT  - European cytokine network
JID - 9100879
RN  - 0 (DNA Primers)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Escherichia coli
MH  - HIV Core Protein p24/biosynthesis
MH  - HIV-1/drug effects/*physiology
MH  - Humans
MH  - Interleukin-18/*pharmacology
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Recombinant Proteins/pharmacology
MH  - Spodoptera
MH  - T-Lymphocytes
MH  - Transfection
MH  - Virus Replication/*drug effects
EDAT- 2000/03/08 00:00
MHDA- 2000/03/08 00:01
CRDT- 2000/03/08 00:00
PHST- 2000/03/08 00:00 [pubmed]
PHST- 2000/03/08 00:01 [medline]
PHST- 2000/03/08 00:00 [entrez]
PST - ppublish
SO  - Eur Cytokine Netw. 2000 Mar;11(1):47-52.