PMID- 10706680
OWN - NLM
STAT- MEDLINE
DCOM- 20000328
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 6
DP  - 2000 Mar 15
TI  - Induction of rapid T cell activation, division, and recirculation by 
      intratracheal injection of dendritic cells in a TCR transgenic model.
PG  - 2937-46
AB  - Dendritic cells (DCs) are thought to be responsible for sensitization to inhaled 
      Ag and induction of adaptive immunity in the lung. The characteristics of T cell 
      activation in the lung were studied after transfer of Ag-pulsed bone 
      marrow-derived DCs into the airways of naive mice. Cell division of Ag-specific T 
      cells in vivo was followed in a carboxyfluorescein diacetate succinimidyl 
      ester-labeled cohort of naive moth cytochrome c-reactive TCR transgenic T cells. 
      Our adoptive transfer system was such that transferred DCs were the only cells 
      expressing the MHC molecule required for presentation of cytochrome c to 
      transgenic T cells. Ag-specific T cell activation and proliferation occurred 
      rapidly in the draining lymph nodes of the lung, but not in nondraining lymph 
      nodes or spleen. No bystander activation of non-Ag-specific T cells was induced. 
      Division of Ag-specific T cells was accompanied by transient expression of CD69, 
      while up-regulation of CD44 increased with each cell division. Divided cells had 
      recirculated to nondraining lymph nodes and spleen by day 4 of the response. In 
      vitro restimulation with specific Ag revealed that T cells were primed to 
      proliferate more strongly and to produce higher amounts of cytokines per cell. 
      These data are consistent with the notion that DCs in the lung are extremely 
      efficient in selecting Ag-reactive T cells from a diverse repertoire. The 
      response is initially localized in the mediastinal lymph nodes, but subsequently 
      spreads systemically. This system should allow us to study the early events 
      leading to sensitization to inhaled Ag.
FAU - Lambrecht, B N
AU  - Lambrecht BN
AD  - Centenary Institute of Cancer Medicine and Cell Biology, Newtown, Sydney, 
      Australia. lambrecht@lond.azr.nl
FAU - Pauwels, R A
AU  - Pauwels RA
FAU - Fazekas De St Groth, B
AU  - Fazekas De St Groth B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytochrome c Group)
RN  - 0 (H-2 Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (I-E-antigen)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Administration, Intranasal
MH  - Adoptive Transfer
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Division/genetics/immunology
MH  - Cell Movement/genetics/*immunology
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Cytochrome c Group/immunology
MH  - Dendritic Cells/immunology/*transplantation
MH  - H-2 Antigens/genetics
MH  - Histocompatibility Antigens Class II/genetics
MH  - Immunophenotyping
MH  - Intubation, Intratracheal
MH  - Lymph Nodes/cytology/immunology
MH  - *Lymphocyte Activation/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Models, Immunological
MH  - Molecular Sequence Data
MH  - Moths
MH  - Peptide Fragments/administration & dosage/immunology
MH  - Receptors, Antigen, T-Cell, alpha-beta/*genetics
MH  - T-Lymphocyte Subsets/*cytology/*immunology/metabolism
EDAT- 2000/03/08 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/03/08 09:00
PHST- 2000/03/08 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/03/08 09:00 [entrez]
AID - ji_v164n6p2937 [pii]
AID - 10.4049/jimmunol.164.6.2937 [doi]
PST - ppublish
SO  - J Immunol. 2000 Mar 15;164(6):2937-46. doi: 10.4049/jimmunol.164.6.2937.