PMID- 10706711
OWN - NLM
STAT- MEDLINE
DCOM- 20000328
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 6
DP  - 2000 Mar 15
TI  - Tumor rejection and immune memory elicited by locally released LEC chemokine are 
      associated with an impressive recruitment of APCs, lymphocytes, and granulocytes.
PG  - 3200-6
AB  - The human beta chemokine known as LEC (also called NCC-4, HCC-4, or LMC) displays 
      chemotactic activity for monocytes and dendritic cells. The possibility that its 
      local presence increases tumor immunogenicity is addressed in this paper. TSA 
      parental cells (TSA-pc) are poorly immunogenic adenocarcinoma cells that grow 
      progressively, kill both nu/nu and syngeneic BALB/c mice, and give rise to lung 
      metastases. TSA cells engineered to release LEC (TSA-LEC) are still able to grow 
      in nu/nu mice, but are promptly rejected and display a marginal metastatic 
      phenotype in BALB/c mice. Rejection is associated with a marked T lymphocyte and 
      granulocyte infiltration, along with extensive macrophage and dendritic cell 
      recruitment. NK cells and CD4+ T lymphocytes are uninfluential in TSA-LEC cell 
      rejection, whereas both CD8+ lymphocytes and polymorphonuclear leukocytes play a 
      major role. An antitumor immune memory is established very quickly after 
      rejection, since 6 days later 75% of BALB/c mice were already resistant to a 
      TSA-pc challenge. Spleen cells from rejecting mice display specific cytotoxic 
      activity against TSA-pc and secrete IFN-gamma and IL-2 when restimulated by 
      TSA-pc. The ability of LEC to markedly improve recognition of poorly immunogenic 
      cells by promoting APC-T cell cross-talk suggests that it could be an effective 
      component of antitumor vaccines.
FAU - Giovarelli, M
AU  - Giovarelli M
AD  - Department of Clinical and Biological Sciences, University of Torino, Orbassano, 
      Italy. mirella.giovarelli@unito.it
FAU - Cappello, P
AU  - Cappello P
FAU - Forni, G
AU  - Forni G
FAU - Salcedo, T
AU  - Salcedo T
FAU - Moore, P A
AU  - Moore PA
FAU - LeFleur, D W
AU  - LeFleur DW
FAU - Nardelli, B
AU  - Nardelli B
FAU - Di Carlo, E
AU  - Di Carlo E
FAU - Lollini, P L
AU  - Lollini PL
FAU - Ruben, S
AU  - Ruben S
FAU - Ullrich, S
AU  - Ullrich S
FAU - Garotta, G
AU  - Garotta G
FAU - Musiani, P
AU  - Musiani P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CCL16 protein, human)
RN  - 0 (Chemokines, CC)
SB  - IM
MH  - Adenocarcinoma/immunology/metabolism/pathology
MH  - Animals
MH  - Antigen-Presenting Cells/immunology/*pathology
MH  - Cell Division/immunology
MH  - Cell Movement/*immunology
MH  - Chemokines, CC/metabolism/*physiology
MH  - Female
MH  - Graft Rejection/*immunology/pathology
MH  - Graft vs Host Reaction/immunology
MH  - Granulocytes/immunology/*pathology
MH  - Humans
MH  - *Immunologic Memory
MH  - Lymphocytes/immunology/*pathology
MH  - Mammary Neoplasms, Experimental/*immunology/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Tumor Cells, Cultured
EDAT- 2000/03/08 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/03/08 09:00
PHST- 2000/03/08 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/03/08 09:00 [entrez]
AID - ji_v164n6p3200 [pii]
AID - 10.4049/jimmunol.164.6.3200 [doi]
PST - ppublish
SO  - J Immunol. 2000 Mar 15;164(6):3200-6. doi: 10.4049/jimmunol.164.6.3200.