PMID- 10708546
OWN - NLM
STAT- MEDLINE
DCOM- 20000419
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 269
IP  - 2
DP  - 2000 Mar 16
TI  - Novel priming compounds of cystathionine metabolites on superoxide generation in 
      human neutrophils.
PG  - 297-301
AB  - Human peripheral blood polymorphonuclear leukocytes were preincubated with 
      cystathionine and cystathionine metabolites found in the urine of patients with 
      cystathioninuria. Among the cystathionine metabolites, cystathionine ketimine and 
      N-acetyl-S-(3-oxo-3-carboxy-n-propyl) cysteine (NAc-OCPC) significantly enhanced 
      the N-formylmethionylleucylphenylalanine (fMLP)-induced superoxide generation, 
      but cystathionine, NAc-cystathionine, and cyclothionine did not enhance the 
      superoxide generation. Cystathionine ketimine and NAc-OCPC also enhanced 
      superoxide generation induced by opsonized zymosan (OZ) but not that induced by 
      arachidonic acid (AA) and phorbol 12-myristate 13-acetate (PMA). Superoxide 
      generation induced by cystathionine ketimine and NAc-OCPC was inhibited by 
      genistein, an inhibitor of tyrosine kinase, and was enhanced by 1-(5-isoquinoline 
      sulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein kinase C. 
      Cystathionine ketimine and NAc-OCPC markedly also increased phosphorylation of 
      45-kDa protein in human neutrophils and the phosphorylation depended on the 
      concentrations of cystathionine ketimine and NAc-OCPC. The phosphorylation of 
      45-kDa protein induced by cystathionine ketimine and NAc-OCPC was inhibited by 
      genistein and herbimycin A, inhibitors of tyrosine kinase, but was not inhibited 
      by H-7 and staurosporine, inhibitors of protein kinase C. Cystathionine 
      metabolites and l-cystathionine sulfoxides were separated into two 
      diastereoisomers, CS-I and CS-II. CS-I enhanced the superoxide generation induced 
      by AA and PMA but not that induced by fMLP and OZ. In contrast, CS-II enhanced 
      the superoxide generation induced by fMLP and OZ, but not that induced by AA and 
      PMA.
CI  - Copyright 2000 Academic Press.
FAU - Kodama, H
AU  - Kodama H
AD  - Department of Chemistry, Kochi Medical School, Oko-cho, Nankoku-shi, Kochi, 
      783-8505, Japan.
FAU - Zhang, J
AU  - Zhang J
FAU - Sugahara, K
AU  - Sugahara K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amino Acids)
RN  - 0 (Carboxylic Acids)
RN  - 0 (N-acetyl-S-(3-oxo-3-carboxy-n-propyl)cysteine)
RN  - 11062-77-4 (Superoxides)
RN  - 375YFJ481O (Cystathionine)
RN  - 42HK56048U (Tyrosine)
RN  - 54927-81-0 (cystathionine sulfoxide)
RN  - 87254-95-3 (cystathionine ketimine)
SB  - IM
MH  - Amino Acids/pharmacology
MH  - Carboxylic Acids/pharmacology
MH  - Cystathionine/analogs & derivatives/*metabolism/pharmacology
MH  - Humans
MH  - Neutrophils/*drug effects/metabolism
MH  - Phosphorylation
MH  - Stereoisomerism
MH  - Superoxides/*metabolism
MH  - Tyrosine/metabolism
EDAT- 2000/03/10 09:00
MHDA- 2000/04/25 09:00
CRDT- 2000/03/10 09:00
PHST- 2000/03/10 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/03/10 09:00 [entrez]
AID - S0006-291X(99)91970-8 [pii]
AID - 10.1006/bbrc.1999.1970 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Mar 16;269(2):297-301. doi: 
      10.1006/bbrc.1999.1970.