PMID- 10708548
OWN - NLM
STAT- MEDLINE
DCOM- 20000419
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 269
IP  - 2
DP  - 2000 Mar 16
TI  - Cell to cell interaction between mesangial cells and macrophages induces the 
      expression of monocyte chemoattractant protein-1 through nuclear factor-kappaB 
      activation.
PG  - 309-16
AB  - The interaction between mesangial cells (MCs) and monocytes/macrophages (Mo/Mo) 
      is an important pathogenic feature of glomerulonephritis. However, its mechanism 
      is not fully elucidated. Studies to date have focused on the interactions through 
      mediators. In the present study, to obtain insight into the mechanism of the 
      interaction between MCs and Mo/Mo, we examined the significance of the cell to 
      cell interaction of these cells in the context of monocyte chemoattractant 
      protein-1 (MCP-1) expression using cell contact cultures or co-culture without 
      contact. Our results revealed that the cellular adhesion of cultured macrophages 
      to MCs induced the expression of MCP-1, which was mainly observed in the MCs. In 
      addition, the induction of MCP-1 was, at least in part, mediated by nuclear 
      factor kappa-B activation which occurs preferentially in the MCs. Because MCP-1 
      is suggested to play an important role in glomerulonephritis, this novel cell to 
      cell interaction between the MCs and Mo/Mo could be important in 
      glomerulonephritis.
CI  - Copyright 2000 Academic Press.
FAU - Hisada, Y
AU  - Hisada Y
AD  - Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka, 
      532-8505, Japan. y-hisada@tanabe.co.jp
FAU - Sakurai, H
AU  - Sakurai H
FAU - Sugaya, T
AU  - Sugaya T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA Primers)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - *Cell Adhesion
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Coculture Techniques
MH  - DNA Primers
MH  - Genes, Reporter
MH  - Glomerular Mesangium/*cytology
MH  - Glomerulonephritis/pathology
MH  - In Situ Hybridization
MH  - Luciferases/genetics
MH  - Macrophages/*cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/*metabolism
MH  - RNA, Messenger/genetics
EDAT- 2000/03/10 09:00
MHDA- 2000/04/25 09:00
CRDT- 2000/03/10 09:00
PHST- 2000/03/10 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/03/10 09:00 [entrez]
AID - S0006-291X(00)92297-6 [pii]
AID - 10.1006/bbrc.2000.2297 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Mar 16;269(2):309-16. doi: 
      10.1006/bbrc.2000.2297.