PMID- 10709111
OWN - NLM
STAT- MEDLINE
DCOM- 20000323
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 85
IP  - 6
DP  - 2000 Mar 15
TI  - Expression analysis of endogenous menin, the product of the multiple endocrine 
      neoplasia type 1 gene, in cell lines and human tissues.
PG  - 877-81
AB  - We have investigated the endogenous expression of menin, a protein encoded by the 
      gene mutated in multiple endocrine neoplasia type 1 (MEN1). Western blot analysis 
      showed strong expression of menin as a 68 kDa protein in all of 7 human and 
      primate cell lines tested. In a panel of 12 fetal human tissue extracts, 68 kDa 
      menin was readily detected in brain cortex, kidney, pituitary, testis and thymus 
      and weakly detected in thyroid. Reproducible bands other than 68 kDa were 
      observed in adrenal and heart, whereas menin was undetectable in liver, lung, 
      pancreas and skin. Analysis of synchronized HeLa cells revealed no variation in 
      the amount or size of menin throughout the cell cycle. Protein expression was 
      compared between lymphoblastoid cell lines from healthy controls and MEN1 
      patients carrying nonsense mutations on 1 allele. No truncated protein was 
      detected in either cytoplasmic or nuclear fractions in mutation-carrying cells. 
      The expression level and cellular location of full-length menin did not differ 
      between cell lines derived from MEN1 patients and healthy donors. This suggests 
      that the wild-type allele has been up-regulated in mutation-carrying cells to 
      compensate for the loss of 1 functional allele.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Wautot, V
AU  - Wautot V
AD  - Laboratoire de Genetique et Cancer, CNRS-UMR 5641, Faculte de Medecine, 
      Universite Claude Bernard, Lyon, France.
FAU - Khodaei, S
AU  - Khodaei S
FAU - Frappart, L
AU  - Frappart L
FAU - Buisson, N
AU  - Buisson N
FAU - Baro, E
AU  - Baro E
FAU - Lenoir, G M
AU  - Lenoir GM
FAU - Calender, A
AU  - Calender A
FAU - Zhang, C X
AU  - Zhang CX
FAU - Weber, G
AU  - Weber G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Cycle
MH  - Fetus
MH  - Gene Expression
MH  - Humans
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - Mutation
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - Primates
MH  - *Proto-Oncogene Proteins
MH  - Reference Values
MH  - Tumor Cells, Cultured
EDAT- 2000/03/10 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/03/10 09:00
PHST- 2000/03/10 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/03/10 09:00 [entrez]
AID - 10.1002/(SICI)1097-0215(20000315)85:6<877::AID-IJC23>3.0.CO;2-F [pii]
AID - 10.1002/(sici)1097-0215(20000315)85:6<877::aid-ijc23>3.0.co;2-f [doi]
PST - ppublish
SO  - Int J Cancer. 2000 Mar 15;85(6):877-81. doi: 
      10.1002/(sici)1097-0215(20000315)85:6<877::aid-ijc23>3.0.co;2-f.