PMID- 10710506
OWN - NLM
STAT- MEDLINE
DCOM- 20000524
LR  - 20181130
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 278
IP  - 3
DP  - 2000 Mar
TI  - Cortisol inhibits hepatocyte growth factor/scatter factor expression and induces 
      c-met transcripts in osteoblasts.
PG  - E509-15
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is expressed by osteoblasts and 
      has important effects on repair and bone remodeling. Because glucocorticoids 
      regulate these two functions, we tested the effects of cortisol on the expression 
      of HGF/SF and c-met, the protooncogene encoding the HGF/SF receptor, in cultures 
      of osteoblast-enriched cells from 22-day fetal rat calvariae (Ob cells). Cortisol 
      decreased HGF/SF mRNA levels and diminished the induction of HGF/SF transcripts 
      by fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor BB (PDGF 
      BB). Cortisol also decreased FGF-2 and PDGF BB-induced HGF/SF mRNA and 
      polypeptide levels in MC3T3 cells. In contrast, cortisol enhanced the expression 
      of c-met transcripts in Ob cells. Cortisol did not modify the half-life of HGF/SF 
      or of c-met mRNA in transcriptionally arrested cells, and it increased the rate 
      of transcription of c-met. In conclusion, cortisol decreases HGF/SF transcripts 
      in Ob cells and enhances c-met expression transcriptionally. The effects of 
      cortisol on HGF/SF could be relevant to its inhibitory actions on bone formation 
      and repair.
FAU - Blanquaert, F
AU  - Blanquaert F
AD  - Departments of Research and Medicine, Saint Francis Hospital and Medical Center, 
      Hartford, CT 06105, USA.
FAU - Pereira, R C
AU  - Pereira RC
FAU - Canalis, E
AU  - Canalis E
LA  - eng
GR  - DK-45227/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (RNA, Messenger)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 1B56C968OA (Becaplermin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Becaplermin
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Hepatocyte Growth Factor/*antagonists & inhibitors/genetics
MH  - Hydrocortisone/*pharmacology
MH  - Osteoblasts/*drug effects/metabolism/*physiology
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Proto-Oncogene Proteins c-met/*genetics
MH  - Proto-Oncogene Proteins c-sis
MH  - RNA, Messenger/antagonists & inhibitors/metabolism
MH  - Rats
MH  - Skull/cytology/embryology
MH  - Transcription, Genetic/*drug effects
EDAT- 2000/03/11 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/03/11 09:00
PHST- 2000/03/11 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/03/11 09:00 [entrez]
AID - 10.1152/ajpendo.2000.278.3.E509 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2000 Mar;278(3):E509-15. doi: 
      10.1152/ajpendo.2000.278.3.E509.