PMID- 10712345
OWN - NLM
STAT- MEDLINE
DCOM- 20000425
LR  - 20041117
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 161
IP  - 3 Pt 1
DP  - 2000 Mar
TI  - Sensitive identification of mycobacterial species using PCR-RFLP on bronchial 
      washings.
PG  - 930-4
AB  - In 98 patients (24 with active pulmonary tuberculosis [TB] lesions, 28 with cured 
      TB lesions, and 46 with nontuberculous opacities [control group] in chest CT 
      scans), we examined whether washing the bronchus after brushing the lesion, then 
      applying polymerase chain reaction-restriction fragment length polymorphism 
      (PCR-RFLP) to the bronchial washings might be useful for diagnosing TB and 
      nontuberculous mycobacteriosis (NTMosis). After biopsy and brushing with a 
      bronchoscope, the bronchus connecting to the lesion was washed with 20 ml saline. 
      The saline used for washing the brushes (5 ml; brushing sample), and 3 to 10 ml 
      saline aspirated through the forceps channel (washing sample) were examined by 
      PCR-RFLP, which proved able to identify Mycobacterium tuberculosis and seven 
      species of nontuberculous mycobacteria (NTM). The values obtained for the 
      sensitivity of the PCR-RFLP with respect to the brushing sample, the washing 
      sample, and both samples mixed together were 70, 76, and 91%, respectively, when 
      only patients who were culture-positive or radiologically improved after 
      antituberculous therapy were considered as showing true infection. A mixture of 
      brushing and washing samples provides useful material for PCR and culture, and 
      the PCR-RFLP used here is a good method for the simultaneous identification of 
      several species of mycobacterium (including M. tuberculosis).
FAU - Hidaka, E
AU  - Hidaka E
AD  - Departments of Laboratory Medicine and Internal Medicine, Shinshu University 
      School of Medicine, Matsumoto, Japan.
FAU - Honda, T
AU  - Honda T
FAU - Ueno, I
AU  - Ueno I
FAU - Yamasaki, Y
AU  - Yamasaki Y
FAU - Kubo, K
AU  - Kubo K
FAU - Katsuyama, T
AU  - Katsuyama T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bronchoalveolar Lavage Fluid/*microbiology
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium avium Complex/genetics
MH  - Mycobacterium avium-intracellulare Infection/diagnosis/microbiology
MH  - Mycobacterium tuberculosis/genetics
MH  - *Polymerase Chain Reaction
MH  - *Polymorphism, Restriction Fragment Length
MH  - Predictive Value of Tests
MH  - Tuberculosis, Pulmonary/*diagnosis/microbiology
EDAT- 2000/03/11 09:00
MHDA- 2000/04/29 09:00
CRDT- 2000/03/11 09:00
PHST- 2000/03/11 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 2000/03/11 09:00 [entrez]
AID - 10.1164/ajrccm.161.3.9904028 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2000 Mar;161(3 Pt 1):930-4. doi: 
      10.1164/ajrccm.161.3.9904028.