PMID- 10712647
OWN - NLM
STAT- MEDLINE
DCOM- 20000525
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 12
IP  - 2
DP  - 2000 Feb
TI  - In vivo survival requirement of a subset of nodose ganglion neurons for nerve 
      growth factor.
PG  - 670-6
AB  - The sensory neurons of the nodose ganglion are the classic example of a 
      population of peripheral nervous system neurons that do not require nerve growth 
      factor (NGF) for survival during development but are dependent on other 
      neurotrophins. We have re-examined this assertion by studying the development of 
      the nodose ganglion of mice that have a null mutation in the NGF gene. Compared 
      with wild-type embryos, the number of neurons undergoing apoptosis was elevated 
      in NGF -/- mice, resulting in a significant reduction in the total number of 
      neurons in the ganglion by the end of embryonic development. TrkA, the NGF 
      receptor tyrosine kinase, was expressed in the nodose ganglion throughout 
      development and there was a marked decrease in TrkA mRNA expression in the nodose 
      ganglion of NGF -/- embryos. Although the in vitro survival of the majority of 
      nodose neurons was promoted by brain-derived neurotrophic factor (BDNF), a minor 
      proportion was supported by NGF in cultures established over a range of embryonic 
      stages. These results clearly demonstrate that a subset of nodose ganglion 
      neurons depends on NGF for survival during development. The finding that the 
      expression of tyrosine hydroxylase (TH) mRNA was unaffected in the nodose ganglia 
      of NGF-deficient embryos indicates that this NGF-dependent subset is distinct 
      from the subset of catacholaminergic neurons in the nodose ganglion.
FAU - Forgie, A
AU  - Forgie A
AD  - School of Biomedical Sciences, Bute Medical Buildings, University of St Andrews, 
      St. Andrews, Fife KY16 9AT, Scotland, UK.
FAU - Kuehnel, F
AU  - Kuehnel F
FAU - Wyatt, S
AU  - Wyatt S
FAU - Davies, A M
AU  - Davies AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.7.10.1 (Receptor, trkA)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Enzyme Induction
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis/genetics
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Growth Factor/deficiency/genetics/pharmacology/*physiology
MH  - Nerve Tissue Proteins/biosynthesis/genetics
MH  - Neurons, Afferent/cytology/*drug effects/physiology
MH  - Nodose Ganglion/*cytology/embryology
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, trkA/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tyrosine 3-Monooxygenase/biosynthesis/genetics
EDAT- 2000/03/11 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/03/11 09:00
PHST- 2000/03/11 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/03/11 09:00 [entrez]
AID - ejn951 [pii]
AID - 10.1046/j.1460-9568.2000.00951.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2000 Feb;12(2):670-6. doi: 10.1046/j.1460-9568.2000.00951.x.