PMID- 10715550
OWN - NLM
STAT- MEDLINE
DCOM- 20000623
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 160
IP  - 1-2
DP  - 2000 Feb 25
TI  - Cyclic AMP stimulates the gene expression of a non-selective cation channel, 
      mNSC1, in pancreatic beta-cell line, MIN6.
PG  - 165-71
AB  - Mouse non-selective cation channel 1 (mNSC 1) cDNA from mouse pancreatic 
      beta-cell line, MIN6, have recently been cloned. Since the number of 
      non-selective cation channel in pancreatic duct cells has been reported to 
      increase 9-fold in 5 h incubation with cAMP, the effect of cAMP on the gene 
      expression of mNSC1 in MIN6 cells was examined. Dibutyryl cAMP (db-cAMP) was 
      shown to increase the level of the mRNA by reverse transcription-polymerase chain 
      reaction (RT-PCR). The copy number of the mRNA was increased 4-fold in 6 h 
      incubation with db-cAMP by competitive PCR. Western blot analysis also indicated 
      a 4-fold increase in the quantity of the newly synthesized protein in 9 h 
      incubation with db-cAMP. Experiments with 5'-flanking region and with a 
      transcriptional inhibitor suggested that db-cAMP affected transcription, and 
      protected the mRNA from its degradation as well. It is concluded that the 
      expression of mNSC1 is indeed increased by cAMP in the pancreatic beta-cells.
FAU - Satoh, J
AU  - Satoh J
AD  - Department of Pharmacology, Jichi Medical School, 3311-1 Yakushiji, 
      Minamikawachi, Kawachi, Tochigi, Japan.
FAU - Kutsuwada, K
AU  - Kutsuwada K
FAU - Ohki, G
AU  - Ohki G
FAU - Imai, M
AU  - Imai M
FAU - Kobayashi, M
AU  - Kobayashi M
FAU - Suzuki, M
AU  - Suzuki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (DNA Primers)
RN  - 0 (Ion Channels)
RN  - 0 (RNA, Messenger)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (Nsccn1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cyclic AMP/*pharmacology
MH  - DNA Primers/genetics
MH  - Gene Expression/drug effects
MH  - Half-Life
MH  - Histone-Lysine N-Methyltransferase
MH  - Ion Channels/*genetics
MH  - Islets of Langerhans/*drug effects/*metabolism
MH  - Mice
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2000/03/15 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/03/15 09:00
PHST- 2000/03/15 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/03/15 09:00 [entrez]
AID - S0303720799002142 [pii]
AID - 10.1016/s0303-7207(99)00214-2 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2000 Feb 25;160(1-2):165-71. doi: 
      10.1016/s0303-7207(99)00214-2.