PMID- 10715806
OWN - NLM
STAT- MEDLINE
DCOM- 20000331
LR  - 20191025
IS  - 0954-6928 (Print)
IS  - 0954-6928 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Feb
TI  - Genetic polymorphism of 5,10-methylenetetrahydrofolate increases risk of 
      myocardial infarction and is correlated to elevated levels of homocysteine in the 
      Japanese general population.
PG  - 47-51
AB  - BACKGROUND: Hyperhomocysteinemia, an independent and graded risk factor for 
      coronary artery disease, can result from both environmental and hereditary 
      factors. C677T mutation of the 5,10-methylenetetrahydrofolate reductase (MTHFR) 
      gene [alanine/valine (A/V) polymorphism], one of the key enzymes involved in 
      catalyzing the remethylation of homocysteine, has recently been reported. 
      OBJECTIVE: To evaluate the incidence of the MTHFR genotypes and their 
      significance in determining the risk for myocardial infarction of Japanese men. 
      METHOD: The subjects consisted of 199 healthy men (mean age, 60 years) and 230 
      male patients with myocardial infarction (mean age, 59 years). The 
      coronary-artery lesions were evaluated by coronary angiography. The MTHFR 
      genotype was analyzed by polymerase chain reaction and then by digestion with 
      Hinfl. Total plasma levels of homocysteine for each MTHFR genotype were compared 
      with those in healthy controls. RESULTS: The prevalences of the A and V alleles 
      among the healthy male subjects were 0.652 and 0.348 in the Hardy-Weinberg 
      equilibrium. The total levels of homocysteine in the plasma of the healthy male 
      subjects were 8.6 +/- 3.3, 8.9 +/- 4.1, and 11.6 +/- 5.6 mumol/l, for AA, AV, and 
      VV genotypes, respectively. Individuals with the VV homozygous mutant genotype 
      thus had the highest plasma levels of homocysteine. Logistic analysis revealed 
      that the levels of high-density lipoprotein cholesterol, hypertension, diabetes 
      mellitus, MTHFR VV genotype, and triglycerides were all independent risk factors 
      for myocardial infarction. The VV genotype was more prevalent among patients with 
      myocardial infarction (mean age, 59 years) than it was among the control subjects 
      (17.0 versus 10.6%, P < 0.05). However, there were no differences in the numbers 
      of stenotic coronary arteries among the MTHFR genotypes. CONCLUSION: The VV 
      genotype of MTHFR increases plasma levels of homocysteine in healthy controls, 
      and this mutation indicates a genetic predisposition toward a greater than normal 
      risk of myocardial infarction for Japanese men.
FAU - Nakai, K
AU  - Nakai K
AD  - Second Department of Internal Medicine & Memorial Heart Center, Iwate Medical 
      University, Tokyo, Japan. knakai@iwate-med.ac.jp
FAU - Fusazaki, T
AU  - Fusazaki T
FAU - Suzuki, T
AU  - Suzuki T
FAU - Ohsawa, M
AU  - Ohsawa M
FAU - Ogiu, N
AU  - Ogiu N
FAU - Kamata, J
AU  - Kamata J
FAU - Kawazoe, K
AU  - Kawazoe K
FAU - Nakai, K
AU  - Nakai K
FAU - Itoh, C
AU  - Itoh C
FAU - Yanagisawa, M
AU  - Yanagisawa M
FAU - Ishida, T
AU  - Ishida T
FAU - Hiramori, K
AU  - Hiramori K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Coron Artery Dis
JT  - Coronary artery disease
JID - 9011445
RN  - 0 (Vitamins)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 1.5.1.5 (Methylenetetrahydrofolate Dehydrogenase (NADP))
SB  - IM
MH  - Cohort Studies
MH  - Homocysteine/adverse effects/*blood
MH  - Humans
MH  - Japan
MH  - Male
MH  - Methylenetetrahydrofolate Dehydrogenase (NADP)/*genetics
MH  - Middle Aged
MH  - Myocardial Infarction/blood/*genetics/prevention & control
MH  - Polymerase Chain Reaction
MH  - Risk Factors
MH  - Vitamins/therapeutic use
EDAT- 2000/03/15 00:00
MHDA- 2000/03/15 00:01
CRDT- 2000/03/15 00:00
PHST- 2000/03/15 00:00 [pubmed]
PHST- 2000/03/15 00:01 [medline]
PHST- 2000/03/15 00:00 [entrez]
AID - 10.1097/00019501-200002000-00009 [doi]
PST - ppublish
SO  - Coron Artery Dis. 2000 Feb;11(1):47-51. doi: 10.1097/00019501-200002000-00009.