PMID- 10718214
OWN - NLM
STAT- MEDLINE
DCOM- 20000329
LR  - 20191103
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 9
IP  - 1
DP  - 2000 Mar
TI  - Genetic imbalances in primary gastric diffuse large B-cell lymphomas: comparison 
      of comparative genomic hybridization, microsatellite, and cytogenetic analysis.
PG  - 58-65
AB  - Extranodal malignant non-Hodgkin's lymphomas account for about 40% of lymphoid 
      neoplasms, but few data are available concerning the genetic background of 
      primary gastric diffuse large B-cell lymphoma (DLBCL). A study was performed of 
      27 primary gastric DLBCLs and 5 gastric DLBCLs with a concomitant low grade 
      component of mucosa-associated lymphoid tissue-type lymphoma using comparative 
      genomic hybridization (CGH), microsatellite studies, classic cytogenetics, and 
      fluorescence in situ hybridization (FISH) to search for specific genetic 
      aberrations. The most frequent aberrations were losses of material on chromosome 
      6q and gains of parts of chromosome 3. In three cases, a total of six high level 
      DNA amplifications were detected, with five of them involving chromosomal regions 
      not having been reported before in gastric DLBCL. A high overall concordance of 
      91.4% between microsatellite analysis and CGH was observed using DNA extracted 
      from the same tissue block. The concordance achieved using DNA from different 
      tissue blocks of the same patient was 85%. Microsatellite studies, CGH, FISH, and 
      classic cytogenetics represent complementary techniques that facilitate a 
      comprehensive view of genetic alterations in malignancies such as primary gastric 
      DLBCL.
FAU - Peters, K
AU  - Peters K
AD  - Institute of Pathology, University of Wurzburg, Germany.
FAU - Zettl, A
AU  - Zettl A
FAU - Starostik, P
AU  - Starostik P
FAU - Greiner, A
AU  - Greiner A
FAU - Rosenwald, A
AU  - Rosenwald A
FAU - Katzenberger, T
AU  - Katzenberger T
FAU - Ott, G
AU  - Ott G
FAU - Muller-Hermelink, H K
AU  - Muller-Hermelink HK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - *Cytogenetic Analysis
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Gene Deletion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymphoma, B-Cell/*genetics/pathology
MH  - Lymphoma, B-Cell, Marginal Zone/genetics/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics/pathology
MH  - Male
MH  - *Microsatellite Repeats
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Stomach Neoplasms/*genetics/pathology
EDAT- 2000/03/16 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/03/16 09:00
PHST- 2000/03/16 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/03/16 09:00 [entrez]
AID - 10.1097/00019606-200003000-00009 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2000 Mar;9(1):58-65. doi: 10.1097/00019606-200003000-00009.