PMID- 10718369
OWN - NLM
STAT- MEDLINE
DCOM- 20000518
LR  - 20181113
IS  - 1071-2690 (Print)
IS  - 1071-2690 (Linking)
VI  - 36
IP  - 2
DP  - 2000 Feb
TI  - Human macrovascular endothelial cells: optimization of culture conditions.
PG  - 125-32
AB  - The purpose of this study is to identify optimal culture conditions to support 
      the proliferation of human macrovascular endothelial cells. Two cell lines were 
      employed: human saphenous vein endothelial cells (HSVEC) and human umbilical vein 
      endothelial cells (HUVEC). The influence of basal nutrient media (14 types), 
      fetal bovine serum (FBS), and mitogens (three types) were investigated in 
      relation to cell proliferation. Additionally, a variety of extracellular matrix 
      (ECM) substrate-coated culture dishes were also tested. The most effective 
      nutrient medium in augmenting cell proliferation was MCDB 131. Compared to the 
      more commonly used M199 medium, MCDB 131 resulted in a 2.3-fold increase in cell 
      proliferation. Media containing 20% FBS increased cell proliferation 7.5-fold 
      compared to serum-free media. Among the mitogens tested, heparin (50 microg/ml) 
      and endothelial cell growth supplement (ECGS) (50 microg/ml) significantly 
      improved cell proliferation. Epithelial growth factor (EGF) provided no 
      improvement in cell proliferation. There were no statistical differences in cell 
      proliferation or morphology when endothelial cells were grown on uncoated culture 
      plates compared to plates coated with ECM proteins: fibronectin, laminin, 
      gelatin, or collagen types I and IV. The culture environment yielding maximal 
      HSVEC and HUVEC proliferation is MCDB 131 nutrient medium supplemented with 2 mM 
      glutamine, 20% FBS, 50 microg/ml heparin, and 50 microg/ml ECGS. The ECM 
      substrate-coated culture dishes offer no advantage.
FAU - Terramani, T T
AU  - Terramani TT
AD  - Department of Surgery, University of Southern California School of Medicine, Los 
      Angeles 90033, USA.
FAU - Eton, D
AU  - Eton D
FAU - Bui, P A
AU  - Bui PA
FAU - Wang, Y
AU  - Wang Y
FAU - Weaver, F A
AU  - Weaver FA
FAU - Yu, H
AU  - Yu H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Culture Media)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Culture Techniques
MH  - Cell Division
MH  - Culture Media
MH  - Endothelium, Vascular/*cytology/metabolism
MH  - Extracellular Matrix/metabolism
MH  - Heparin/pharmacology
MH  - Humans
MH  - Microscopy, Phase-Contrast
MH  - Saphenous Vein/cytology
MH  - Umbilical Veins/cytology
EDAT- 2000/03/16 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/03/16 09:00
PHST- 2000/03/16 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/03/16 09:00 [entrez]
AID - 10.1290/1071-2690(2000)036<0125:HMECOO>2.0.CO;2 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2000 Feb;36(2):125-32. doi: 
      10.1290/1071-2690(2000)036<0125:HMECOO>2.0.CO;2.