PMID- 10719334
OWN - NLM
STAT- MEDLINE
DCOM- 20000511
LR  - 20131121
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 20
IP  - 3
DP  - 2000 Mar
TI  - Choice of anticoagulant can influence the analysis using fluorescence in situ 
      hybridization of fetal cells enriched from maternal blood.
PG  - 257-9
AB  - A notable degree of research attention is being focused on the use of fetal cells 
      enriched from the blood of pregnant women as a non-invasive means of prenatal 
      diagnosis. By using magnetic activated cells sorting (MACS) and fluorescence in 
      situ hybridization (FISH), we have examined the efficacy of enriched fetal cells 
      in determining fetal sex. An unexpected finding of this investigation was that 
      the sensitivity of this analysis was influenced by the anticoagulant used to 
      treat the maternal blood samples. As such, samples treated with heparin showed 
      significantly lower detection rates than samples chelated with EDTA.
CI  - Copyright 2000 John Wiley & Sons, Ltd.
FAU - Brombacher, V
AU  - Brombacher V
AD  - Department of Obstetrics and Gynaecology, University of Basel, Basel, 
      Switzerland.
FAU - Kiefer, V
AU  - Kiefer V
FAU - Troeger, C
AU  - Troeger C
FAU - Vial, Y
AU  - Vial Y
FAU - Minderer, S
AU  - Minderer S
FAU - Holzgreve, W
AU  - Holzgreve W
FAU - Hahn, S
AU  - Hahn S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (Anticoagulants)
RN  - 9005-49-6 (Heparin)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Anticoagulants/*pharmacology
MH  - Blood Cells/cytology/*drug effects
MH  - Blood Specimen Collection/methods
MH  - Cell Death
MH  - Edetic Acid/pharmacology
MH  - Female
MH  - Fetus/*cytology
MH  - Heparin/pharmacology
MH  - Humans
MH  - Immunomagnetic Separation
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Sensitivity and Specificity
EDAT- 2000/03/17 09:00
MHDA- 2000/05/16 09:00
CRDT- 2000/03/17 09:00
PHST- 2000/03/17 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 2000/03/17 09:00 [entrez]
AID - 10.1002/(SICI)1097-0223(200003)20:3<257::AID-PD781>3.0.CO;2-V [pii]
PST - ppublish
SO  - Prenat Diagn. 2000 Mar;20(3):257-9.