PMID- 10719373
OWN - NLM
STAT- MEDLINE
DCOM- 20000518
LR  - 20051117
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 27
IP  - 4
DP  - 2000 Apr
TI  - Spectral karyotyping combined with locus-specific FISH simultaneously defines 
      genes and chromosomes involved in chromosomal translocations.
PG  - 418-23
AB  - Genes that play roles in malignant transformation have often been found proximate 
      to cancer-associated chromosomal breakpoints. Identifying genes that flank 
      chromosomal reconfigurations is thus essential for cancer cytogenetics. To 
      simplify and expedite this identification, we have developed a novel approach, 
      based on simultaneous spectral karyotyping and fluorescence in situ hybridization 
      (FISH) which, in a single step, can identify gross chromosomal aberrations as 
      well as detect the involvement of specific loci in these rearrangements. Signals 
      for specifically queried genes (FISH probe) were easily detectable in metaphase 
      cells, together with the signals from painted chromosomes (spectral karyotyping 
      probes). The concentration and size of the FISH probes could cover a wide range 
      and still be used successfully. Some of the nucleotide-bound dyes used for the 
      labeling, as Cy3, Spectrum Orange, Alexa 594, Texas Red, and Rhodamine 110, were 
      particularly efficient. More than one gene can be queried in the same metaphase, 
      because multiple FISH probes could be hybridized simultaneously. To demonstrate 
      this technique, we applied it to the myeloma cell line Karpas 620, which has 
      numerous chromosomal rearrangements. The approach that we present here will be 
      particularly useful for the analysis of complex karyotypes and for testing 
      hypotheses arising from cancer gene expression studies. Published 2000 
      Wiley-Liss, Inc.
FAU - Tonon, G
AU  - Tonon G
AD  - Genetics Department, Medicine Branch, National Cancer Institute, Bethesda, MD 
      20889-5105, USA.
FAU - Roschke, A
AU  - Roschke A
FAU - Stover, K
AU  - Stover K
FAU - Shou, Y
AU  - Shou Y
FAU - Kuehl, W M
AU  - Kuehl WM
FAU - Kirsch, I R
AU  - Kirsch IR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Aberrations/genetics
MH  - DNA Probes/metabolism
MH  - DNA, Neoplasm/metabolism
MH  - Fluorescent Dyes/metabolism
MH  - Genes, Neoplasm/*genetics
MH  - Genetic Markers/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/methods
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2000/03/17 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/03/17 09:00
PHST- 2000/03/17 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/03/17 09:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200004)27:4<418::AID-GCC12>3.0.CO;2-O [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Apr;27(4):418-23.