PMID- 10719762
OWN - NLM
STAT- MEDLINE
DCOM- 20000328
LR  - 20190513
IS  - 1079-5006 (Print)
IS  - 1079-5006 (Linking)
VI  - 55
IP  - 1
DP  - 2000 Jan
TI  - Effect of long-term caloric restriction on GLUT4, phosphatidylinositol-3 kinase 
      p85 subunit, and insulin receptor substrate-1 protein levels in rhesus monkey 
      skeletal muscle.
PG  - B44-6; discussion B47-8
AB  - Caloric restriction (CR), a reduction in calorie intake without malnutrition, 
      improves insulin sensitivity in various species, including mice, rats, rhesus and 
      cynomolgus monkeys, and humans. Skeletal muscle is quantitatively the most 
      important tissue for blood glucose clearance. Therefore, we assessed the effect 
      of 6 years of CR (30% reduction in calorie intake) in male rhesus monkeys (14-20 
      years old) on muscle expression of several proteins involved in insulin action. 
      Whole body insulin sensitivity (assessed by Modified Minimal Model) was 
      significantly increased in CR relative to Control monkeys. CR did not alter the 
      expression of GLUT4 glucose transporter or phosphatidylinositol-3 kinase p85 
      subunit (PI3K). Insulin receptor substrate-1(IRS-1) abundance tended to be 
      greater for CR compared to Control monkeys (p = .051), but correlational analysis 
      revealed no association between IRS-1 and insulin sensitivity (r2 = .075, p = 
      .271). These findings indicate that the CR-induced increase in insulin 
      sensitivity in rhesus monkeys is unrelated to alterations in GLUT4, P13K, and 
      IRS-1 abundance.
FAU - Gazdag, A C
AU  - Gazdag AC
AD  - Department of Nutritional Sciences, University of Wisconsin-Madison, 53706, USA.
FAU - Sullivan, S
AU  - Sullivan S
FAU - Kemnitz, J W
AU  - Kemnitz JW
FAU - Cartee, G D
AU  - Cartee GD
LA  - eng
GR  - P01 AG11915/AG/NIA NIH HHS/United States
GR  - P51 RR00167/RR/NCRR NIH HHS/United States
GR  - T32 AG00213/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Gerontol A Biol Sci Med Sci
JT  - The journals of gerontology. Series A, Biological sciences and medical sciences
JID - 9502837
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Aging/physiology
MH  - Animals
MH  - *Energy Intake
MH  - Glucose Transporter Type 4
MH  - Insulin Resistance
MH  - Macaca mulatta
MH  - Male
MH  - Monosaccharide Transport Proteins/*metabolism
MH  - *Muscle Proteins
MH  - Muscle, Skeletal/*metabolism
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Receptor, Insulin/*metabolism
MH  - Statistics, Nonparametric
EDAT- 2000/03/17 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/03/17 09:00
PHST- 2000/03/17 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/03/17 09:00 [entrez]
AID - 10.1093/gerona/55.1.b44 [doi]
PST - ppublish
SO  - J Gerontol A Biol Sci Med Sci. 2000 Jan;55(1):B44-6; discussion B47-8. doi: 
      10.1093/gerona/55.1.b44.